A two-stage continuous system with two stirred tank reactors in series was utilized to perform syngas fermentation using Clostridium carboxidivorans. The first bioreactor (bioreactor 1) was maintained at pH 6 to promote acidogenesis and the second one (bioreactor 2) at pH 5 to stimulate solventogenesis. Both reactors were operated in continuous mode by feeding syngas (CO:CO₂:H₂:N₂; 30:10:20:40; vol%) at a constant flow rate while supplying a nutrient medium at different flow rates of 8.1, 15, 22 and 30 ml/h. A cell recycling unit was added to bioreactor 2 in order to recycle the cells back to the reactor, maintaining the OD₆₀₀ around 1 in bioreactor 2 throughout the experimental run. When comparing the flow rates, the best results in terms of solvent production were obtained with a flow rate of 22 ml/h, reaching the highest average outlet concentration for alcohols (1.51 g/L) and the most favorable alcohol/acid ratio of 0.32.

利用具有串联的两个搅拌釜反应器的两阶段连续系统，使用碳氧化梭菌（Clostridium carboxidivorans）进行合成气发酵。将第一个生物反应器（生物反应器1）保持在pH 6以促进酸生成，将第二个生物反应器（生物反应器2）保持在pH 5以刺激溶剂生成。两个反应器均通过以恒定流速进料合成气（CO：CO ₂：H ₂：H ₂：N ₂ ; 30：10：20：40; vol％）连续运行，同时以8.1的不同流速供应营养培养基， 15、22和30毫升/小时。将细胞回收单元添加到生物反应器2中，以将细胞回收回反应器，并保持OD ₆₀₀在整个实验过程中，生物反应器2中大约有1个在生物反应器中。在比较流速时，以22 ml / h的流速获得了最佳的溶剂生产结果，达到了最高的醇平均出口浓度（1.51 g / L）和最有利的醇酸比0.32 。