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A highly sensitive and rapidly responding fluorescent probe based on a rhodol fluorophore for imaging endogenous hypochlorite in living mice†
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1039/c7tb02862h
Yanhui Zhang 1, 2, 3, 4 , Lin Ma 5, 6, 7, 8, 9 , Chunchao Tang 5, 6, 7, 8, 9 , Shengnan Pan 1, 2, 3, 4 , Donglei Shi 1, 2, 3, 4 , Shaojing Wang 1, 2, 3, 4 , Minyong Li 5, 6, 7, 8, 9 , Yuan Guo 1, 2, 3, 4
Affiliation  

Hypochlorous (HOCl) acid is generated as a defense tool in the immune system and plays a vital role in killing a wide range of pathogens. There is therefore great interest in developing fluorescent probes that can endogenously respond to the change in concentration of HOCl in vivo. To address this challenge, we here present a rapidly responding fluorescent probe RO610 to image endogenous HOCl in living mice. The development of RO610 was based on a novel water-soluble and pH-independent fluorescent xanthene dye, 2′-formylrhodol ROA, which exhibits highly selective and sensitive responses to HOCl/ClO over other reactive species. Moreover, adding a little more than 5 equiv. of ClO to the solution of RO610 resulted in a clearly observable fluorescence enhancement (48-fold) within 30 s. Based on these properties, RO610 was used to detect ClO in A549 cells without interference by other oxidants. It was applied for the imaging of endogenous HOCl in living nude mice with satisfactory results.

中文翻译:

基于Rhodol荧光团的高灵敏,响应迅速的荧光探针,可对活体小鼠的内源性次氯酸盐成像

次氯酸(HOCl)酸是免疫系统中的防御工具,在杀死各种病原体中起着至关重要的作用。因此,人们对开发能够对体内HOCl浓度内源性反应的荧光探针产生极大的兴趣。为了解决这一挑战,我们在这里提出了一种快速反应的荧光探针RO610,可以对活体小鼠中的内源性HOCl进行成像。的发展RO610是基于一个新的水溶性和pH非依赖性的荧光染料呫吨,2'-formylrhodol ROA,其表现出对次氯酸/ CLO高选择性和灵敏的响应-优于其它反应性物质。此外,增加了超过5当量。的CLO -将其加入RO610溶液可在30 s内明显观察到荧光增强(48倍)。基于这些性质,RO610用于检测CLO -在A549细胞而不通过其它氧化剂的干扰。将其用于活体裸鼠内源性HOCl成像,结果令人满意。
更新日期:2018-01-04
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