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Disposable inkjet-printed electrochemical platform for detection of clinically relevant HER-2 breast cancer biomarker
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2018-01-04 , DOI: 10.1016/j.bios.2018.01.003
Susanita Carvajal , Samantha N. Fera , Abby L. Jones , Thaisa A. Baldo , Islam M. Mosa , James F. Rusling , Colleen E. Krause

Rapidly fabricated, disposable sensor platforms hold tremendous promise for point-of-care detection. Here, we present an inexpensive (< $0.25) fully inkjet printed electrochemical sensor with integrated counter, reference, and working electrodes that is easily scalable for commercial fabrication. The electrochemical sensor platform featured an inkjet printed gold working 8-electrode array (WEA) and counter electrode (CE), along with an inkjet –printed silver electrode that was chlorinated with bleach to produce a Ag/AgCl quasi-reference electrode (RE). As proof of concept, the electrochemical sensor was successfully applied for detection of clinically relevant breast cancer biomarker Human Epidermal Growth Factor Receptor 2 (HER-2). Capture antibodies were bound to a chemically modified surface on the WEA and placed into a microfluidic device. A full sandwich immunoassay was constructed following a simultaneous injection of target protein, biotinylated antibody, and polymerized horseradish peroxide labels into the microfluidic device housing the WEA. With an ultra fast assay time, of only 15 mins a clinically relevant limit of detection of 12 pg mL−1 was achieved. Excellent reproducibility and sensitivity were observed through recovery assays preformed in human serum with recoveries ranging from 76% to 103%. These easily fabricated and scalable electrochemical sensor platforms can be readily adapted for multiplex detection following this rapid assay protocol for cancer diagnostics.



中文翻译:

一次性喷墨打印电化学平台,用于检测临床相关的HER-2乳腺癌生物标志物

快速制造的一次性传感器平台为即时检测提供了广阔的前景。在这里,我们介绍了一种便宜的(<0.25美元)完全喷墨印刷的电化学传感器,带有集成的计数电极,参比电极和工作电极,可轻松扩展用于商业制造。电化学传感器平台具有喷墨印刷的金工作8电极阵列(WEA)和对电极(CE),以及喷墨印刷的银电极,该电极用漂白剂进行氯化处理以生成Ag / AgCl准参比电极(RE) 。作为概念证明,电化学传感器已成功应用于检测临床相关的乳腺癌生物标记物人表皮生长因子受体2(HER-2)。捕获抗体与WEA上经过化学修饰的表面结合,并置于微流控设备中。在将目标蛋白,生物素化抗体和聚合的辣根过氧化物标记物同时注射到装有WEA的微流控设备中之后,构建了全三明治免疫测定法。超快速的分析时间,仅需15分钟即可达到12 pg mL的临床相关检测极限达到-1。通过在人血清中进行的回收测定,观察​​到了极好的可重复性和敏感性,回收率在76%至103%之间。这些易于制造和可扩展的电化学传感器平台可以很容易地按照这种用于癌症诊断的快速测定方案进行多重检测。

更新日期:2018-01-04
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