A versatile platform allowing capture and detection of normal and dysfunctional cells on the same patterned surface is important for accessing the cellular mechanism, developing diagnostic assays, and implementing therapy. Here, an original and effective method for fabricating binary polymer brushes pattern is developed for controlled cell adhesion. The binary polymer brushes pattern, composed of poly(N‐isopropylacrylamide) (PNIPAAm) and poly[poly(ethylene glycol) methyl ether methacrylate] (POEGMA) chains, is simply obtained via a combination of surface‐initiated photopolymerization and surface‐activated free radical polymerization. This method is unique in that it does not utilize any protecting groups or procedures of backfilling with immobilized initiator. It is demonstrated that the precise and well‐defined binary polymer patterns with high resolution are fabricated using this facile method. PNIPAAm chains capture and release cells by thermoresponsiveness, while POEGMA chains possess high capability to capture dysfunctional cells specifically, inducing a switch of normal red blood cells (RBCs) arrays to hemolytic RBCs arrays on the pattern with temperature. This novel platform composed of binary polymer brush pattern is smart and versatile, which opens up pathways to potential applications as microsensors, biochips, and bioassays.

中文翻译：

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易于制备的可控细胞粘附的分层热响应性二元聚合物图案。

一个多功能的平台允许捕获和检测同一图案表面上的正常细胞和功能异常的细胞，对于访问细胞机制，开发诊断分析方法和实施治疗非常重要。在此，开发了一种新颖且有效的制造二元聚合物毛刷图案的方法来控制细胞粘附。二元聚合物画笔图案，由poly（N异丙基丙烯酰胺）（PNIPAAm）和聚[聚（乙二醇）甲基丙烯酸甲酯]（POEGMA）链是通过表面引发的光聚合和表面活化的自由基聚合的简单结合而获得的。该方法的独特之处在于它不使用任何保护基团或固定化引发剂回填的步骤。事实证明，使用这种简便的方法可以制造出具有高分辨率的精确且定义明确的二元聚合物图案。PNIPAAm链通过热响应来捕获和释放细胞，而POEGMA链则具有特异性捕获功能异常的细胞的高能力，从而随着温度的变化，导致正常红细胞（RBC）阵列向溶血性RBC阵列的转换。这个由二元聚合物画笔图案组成的新颖平台既智能又通用，