A novel molecularly imprinted stir bar (MI-SB) for sorptive extraction of semicarbazide (SEM) was prepared in present paper. The coating of the stir bar was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, dynamic adsorption and static adsorption tests. The saturated adsorption of MI-SB was about 4 times over that of non-imprinted stir bar (NI-SB). The selectivity of MI-SB for SEM was much better than NI-SB. A method to determine SEM was established by coupling MI-SB sorptive extraction with HPLC-UV. The liner range was 1–100 ng/mL for SEM with a correlation coefficient of 0.9985. The limit of detection was about 0.59 ng/mL, which was below the minimum required performance limit of SEM in meat products regulated by European Union. The method was applied to the determination of SEM in fish samples with satisfactory results.

本文制备了一种新型的分子印迹搅拌棒（MI-SB），用于吸附萃取氨基脲（SEM）。搅拌棒的涂层通过扫描电子显微镜，傅立叶变换红外光谱，动态吸附和静态吸附测试进行表征。MI-SB的饱和吸附量约为非压印搅拌棒（NI-SB）的4倍。MI-SB对SEM的选择性比NI-SB好得多。通过将MI-SB吸附萃取与HPLC-UV偶联建立了确定SEM的方法。SEM的线性范围为1–100 ng / mL，相关系数为0.9985。检出限约为0.59 ng / mL，低于欧盟规定的肉类产品中SEM的最低性能要求。