Two phase bioreactor was constructed, designed and developed to evaluate the chlorpyrifos remediation. Six biotic and abiotic factors (substrate-loading rate, slurry phase pH, slurry phase dissolved oxygen (DO), soil water ratio, temperature and soil micro flora load) were evaluated by design of experimental (DOE) methodology employing Taguchi's orthogonal array (OA). The selected six factors were considered at two levels L-8 array (2^7, 15 experiments) in the experimental design. The optimum operating conditions obtained from the methodology showed enhanced chlorpyrifos degradation from 283.86 µg/g to 955.364 µg/g by overall 70.34% of enhancement. In the present study, with the help of few well defined experimental parameters a mathematical model was constructed to understand the complex bioremediation process and optimize the approximate parameters upto great accuracy.

建造，设计和开发了两相生物反应器，以评估毒死rif的修复。通过使用田口正交阵列（OA）设计的实验（DOE）方法设计，评估了六个生物和非生物因素（基质负载率，浆相pH，浆相溶解氧（DO），土壤水比，温度和土壤微生物区系负载）。 ）。在实验设计中，在两个级别的L-8阵列（2 ^ 7，15个实验）中考虑了所选的六个因子。从该方法获得的最佳操作条件显示毒死rif降解能力从283.86 µg / g提高到955.364 µg / g，提高了70.34％。在目前的研究中，