Chicken blood contains copious amounts of edible protein, which is currently underutilized. In this study, chicken blood corpuscle was hydrolyzed using two proteases (papain and flavourzyme) and its hydrolysis conditions were optimized by means of response surface methodology (RSM). The optimal conditions based on obtaining a hydrolysate with maximum antioxidant activity were E/S 2.0%, temperature 50 °C, and time 6.0 h. Under the above conditions, the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, superoxide anion scavenging activity and reducing power of papain and flavourzyme hydrolysate (PFH) were 94.99 ± 0.31%, 57.39 ± 2.82%, and 1.83 ± 0.06, respectively. Additionally, PFH showed not only a stable DPPH scavenging activity in the pH ranges of 1 to 7, but also a good tolerance to magnesium (Mg^2 +), potassium (K⁺) and calcium (Ca^2 +). We also found that PFH retained at least 95% of antioxidant capacity under simulated gastrointestinal digestion. Subsequently, PFH was purified sequentially by ultrafiltration, anion exchange chromatography, gel filtration chromatography, and high-performance liquid chromatography (HPLC). Finally, the sequence of the peptide with the highest antioxidant activity was identified to be AEDKKLIQ (943.5 Da) using LC-ESI-MS/MS. Further, this peptide showed free radicals scavenging ability and reducing power as well as that of GSH (P > 0.05), further evidencing its potential used as an antioxidative ingredient.

鸡血中含有大量可食用蛋白质，目前该蛋白质未被充分利用。在这项研究中，使用两种蛋白酶（木瓜蛋白酶和风味酶）水解鸡血小球，并通过响应表面方法（RSM）优化了其水解条件。基于获得具有最大抗氧化活性的水解产物的最佳条件为E / S 2.0％，温度50°C和时间6.0 h。在上述条件下，木瓜蛋白酶和风味酶水解物（PFH）的DPPH（1,1-二苯基-2-甲基苯并肼基）自由基清除活性，超氧阴离子清除活性和还原能力为94.99±0.31％，57.39±2.82％和1.83分别为±0.06。此外，PFH不仅在1至7的pH范围内表现出稳定的DPPH清除活性，而且对镁（Mg ^{2 +}），钾（K ⁺）和钙（Ca ^{2 +}）。我们还发现，在模拟胃肠道消化过程中，PFH保留了至少95％的抗氧化能力。随后，通过超滤，阴离子交换色谱，凝胶过滤色谱和高效液相色谱（HPLC）依次纯化PFH。最后，使用LC-ESI-MS / MS将具有最高抗氧化活性的肽序列鉴定为AEDKKLIQ（943.5 Da）。此外，该肽与GSH相比，具有清除自由基的能力和还原能力（P> 0.05），进一步证明了其作为抗氧化成分的潜力。