当前位置: X-MOL 学术Food Control › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Restricted access supramolecular solvents for the simultaneous extraction and cleanup of ochratoxin A in spices subjected to EU regulation
Food Control ( IF 6 ) Pub Date : 2018-06-01 , DOI: 10.1016/j.foodcont.2018.01.003
Noelia Caballero-Casero , Sergio García-Fonseca , Soledad Rubio

Abstract A simple and high-throughput sample treatment, based on the use of a supramolecular solvent with restricted access properties (SUPRAS-RAM), was developed for the quantification of ochratoxin A (OTA) in spices subjected to EU regulation. The RAM properties of the SUPRAS permitted exclusion of macromolecules by chemical and physical mechanisms, and pigments and essential oils did not influence OTA extraction. Sample treatment took 10 min and required minute amounts of spice (0.2 g) and volume of SUPRAS (0.4 mL). After sample centrifugation, the crude extracts were directed analyzed by liquid chromatography/fluorescence detection (LC-FD) using external calibration. Method validation proved that it met the performance criteria set by the EU guidelines in terms of sensitivity, recoveries, precision, selectivity and trueness. Recoveries found for OTA in spices classified as difficult and unique commodities by the EU (i.e. Capsicum, Piper nutmeg, ginger and turmeric) ranged from 81 to 101%, with repeatability and intermediate precision, expressed as relative standard deviation, in the intervals 0.4–7% and 2–9%, respectively. Method detection and quantification methods were 1.2 and 3 μg/kg. The method meets the requirements to be applied in enforcement and surveillance programs related to OTA in spices.

中文翻译:

用于同时提取和净化受欧盟监管的香料中赭曲霉毒素 A 的限制获取超分子溶剂

摘要 基于使用具有受限访问特性的超分子溶剂 (SUPRAS-RAM),开发了一种简单且高通量的样品处理方法,用于对受欧盟监管的香料中的赭曲霉毒素 A (OTA) 进行定量。SUPRAS 的 RAM 特性允许通过化学和物理机制排除大分子,并且色素和精油不影响 OTA 提取。样品处理需要 10 分钟,需要微量的香料 (0.2 g) 和体积的 SUPRAS (0.4 mL)。样品离心后,使用外部校准通过液相色谱/荧光检测 (LC-FD) 直接分析粗提取物。方法验证证明它在灵敏度、回收率、精密度、选择性和真实性方面符合欧盟指南设定的性能标准。在欧盟归类为困难和独特商品的香料(即辣椒、胡椒肉豆蔻、生姜和姜黄)中发现的 OTA 回收率为 81% 至 101%,具有重复性和中间精度,以相对标准偏差表示,区间为 0.4–分别为 7% 和 2–9%。方法检测和定量方法分别为 1.2 和 3 μg/kg。该方法满足与香料中 OTA 相关的执法和监督计划的要求。
更新日期:2018-06-01
down
wechat
bug