A novel green synthesis strategy was proposed for preparation of multitemplate molecularly imprinted biopolymers (mt-MIBP) in aqueous media with less consumption of organic solvents, which were subsequently used as sorbents of ultrasound-assisted dispersive solid-phase extraction (d-SPE) for simultaneous recognition and efficient separation of B-family vitamins in juice samples, followed by high performance liquid chromatography (HPLC) determination. The obtained mt-MIBP was fully characterized by SEM, FT-IR, TEM, and BET. It offered high binding capacity, good selectivity, and fast dynamics toward all the templates. Involved parameters in the d-SPE efficiency such as mt-MIBP mass, sonication time, and eluting/washing solvents’ types and volumes were concurrently investigated by central composite design with rapidity and reliability. Under the optimum conditions, the developed mt-MIBP-d-SPE-HPLC method exhibited wide linear range, low limits of detection and quantification (LOQs) within 1.2–5.5 μg L^–1 and 4.0–18.4 μg L^–1, respectively, and appropriate repeatability (relative standard deviation values below 4.2%, n = 4). The high selectivity of this method makes it suitable for successful monitoring of vitamins in juice samples with satisfactory recoveries of 75.8–92.7%, 81.1–92.5%, and 84.7–93.8% for vitamins riboflavin (B₂), nicotinamide (B₃), and pyridoxine (B₆), respectively. The present study implied highly promising perspectives of water-compatible eco-friendly mt-MIBP for highly effective multiresidue analysis in complicated matrixes.

中文翻译：

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基于绿色合成策略测定B族维生素的亲水性多模板分子印迹生物聚合物

提出了一种新颖的绿色合成策略，该方法用于在水性介质中以较少的有机溶剂消耗制备多模板分子印迹生物聚合物（mt-MIBP），随后将其用作超声辅助分散固相萃取（d-SPE）的吸附剂。同时识别和有效分离果汁样品中的B族维生素，然后进行高效液相色谱（HPLC）测定。所得的mt-MIBP通过SEM，FT-IR，TEM和BET进行了充分表征。它具有高结合力，良好的选择性和对所有模板的快速动力学。集中复合设计同时研究了d-SPE效率中涉及的参数，例如mt-MIBP的质量，超声处理时间以及洗脱/洗涤溶剂的类型和体积，从而快速，可靠地进行了研究。^{分别为–1}和4.0–18.4μgL ^–1，并具有适当的重复性（相对标准偏差值低于4.2％，n = 4）。该方法的高选择性使其能够成功监测果汁样品中的维生素，其中维生素核黄素（B ₂），烟酰胺（B ₃）的回收率分别为75.8–92.7％，81.1–92.5％和84.7–93.8％，令人满意，和吡ido醇（B ₆）。本研究暗示了与水相容的生态友好型mt-MIBP的前景广阔的前景，可用于复杂基质中的高效多残留分析。