This paper reports the preparation of porous polydimethylsiloxane (PDMS) monolith and its application in protein digestion. Glass microbubbles were used as the template to prepare the porous PDMS monolith. The PDMS monolith presented the interconnected porous structure with a high specific surface area. The average pore size of the porous PDMS monolith was 51 μm with the interconnected pores of 15 μm. Trypsin was immobilized on the inner pore surface of the PDMS monolith with high stability and high density by using the polydopamine as the primer with the advantage of the mild and efficient immobilization condition. Compared with the 12 hour in-solution digestion, the porous PDMS monolith could digest the protein within 15.6 minutes. The high digestion efficiency by continuously flowing the model protein solutions through the porous PDMS monolith with immobilized trypsin demonstrated the value of the porous PDMS monolith as a solid support for enzyme microreactors.

中文翻译：

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多孔聚二甲基硅氧烷整体柱，用于蛋白质消化†

本文报道了多孔聚二甲基硅氧烷（PDMS）整体材料的制备及其在蛋白质消化中的应用。玻璃微泡用作模板以制备多孔PDMS整料。PDMS整体材料提供了具有高比表面积的互连多孔结构。多孔PDMS整料的平均孔径为51μm，相互连通的孔径为15μm。通过使用聚多巴胺作为底漆，胰蛋白酶以温和而有效的固定条件，以高稳定性和高密度将其固定在PDMS整体的内孔表面上。与12小时的溶液内消化相比，多孔PDMS整体可以在15.6分钟内消化蛋白质。