β(1,4)-Galactosyltransferase (β4Gal-T1) and T. cruzi trans-sialidase (TcTS) have been used in a ‘one-pot’ cascade to provide vesicles (liposomes) with a trisaccharide coating. These soluble enzymes catalysed the transfer of galactose then sialic acid onto a synthetic N-acetylglucolipid embedded in the bilayers. Clustering of this substrate into microdomains increased the rate of sialylated lipid production, showing that an increase in β4Gal-T1 activity is carried through the enzymatic cascade. These coatings modulated cell recognition. Hepatocellular carcinoma cells took up vesicles modified by β4Gal-T1 alone more extensively than sialylated vesicles produced by ‘one-pot’ sequential enzymatic modification.

中文翻译：

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囊膜中合成糖脂的“一锅法”顺序酶促修饰

β（1,4）-半乳糖基转移酶（β4Gal-T1）和克鲁氏锥虫反唾液酸酶（TcTS）已用于“一锅”级联反应，以为囊泡（脂质体）提供三糖涂层。这些可溶性酶催化半乳糖然后唾液酸转移到包埋在双层中的合成的N-乙酰葡萄糖脂上。将该底物聚集成微区增加了唾液酸化脂质产生的速率，表明β4Gal-T1活性的增加是通过酶促级联反应进行的。这些涂层调节细胞识别。肝细胞癌细胞吸收的单独受β4Gal-T1修饰的囊泡比通过“一锅法”顺序酶促修饰产生的唾液酸化囊泡更广泛。