Novel magnetic molecularly imprinted nanomaterials (DA + BSA-MMIPs) were prepared adopting bovine serum albumin (BSA) and dopamine as bifunctional monomers for the first time. Besides the role of assistant functional monomer, BSA can exclude the proteins with like charges and promote low molecular weight tetracyclines to be adsorbed. Thus, the DA + BSA-MMIPs could fulfil the selective separation of tetracyclines directly from milk samples. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of uniform imprinting layers, stable crystalline phase, and good magnetism of the DA + BSA-MMIPs, they have rapid binding kinetic, high adsorption capacity, and favorable reusability. The imprinted nanomaterials were coupled with HPLC to selectively extract and determine trace tetracyclines from untreated milk samples. The recoveries of tetracyclines are in the range of 84.1–95.8% with relative standard deviations of less than 6.7%. The developed method is especially suitable for the selective enrichment and detection of target compounds directly from a complex sample with proteins.

首次以牛血清白蛋白（BSA）和多巴胺为双功能单体，制备了新型磁性分子印迹纳米材料（DA + BSA-MMIPs）。除了辅助功能单体的作用外，BSA还可以排除具有类似电荷的蛋白质，并促进低分子量四环素的吸附。因此，DA + BSA-MMIPs可以直接从牛奶样品中选择性分离四环素。详细研究了所得纳米材料的特性，聚合条件和吸附性能。除了具有均匀的压印层，稳定的结晶相和DA + BSA-MMIPs的良好磁性外，它们还具有快速的结合动力学，高吸附能力和良好的可重复使用性。印迹的纳米材料与HPLC结合，从未处理的牛奶样品中选择性提取和测定痕量四环素。四环素的回收率在84.1–95.8％范围内，相对标准偏差小于6.7％。所开发的方法特别适合直接从具有蛋白质的复杂样品中选择性富集和检测目标化合物。