Objective

This aim of this study was to investigate the cell behavioural response on clinically applied dental composites in exposition-relevant target cells, i.e. human gingival fibroblasts (HGF) and epithelial keratinocytes (HGK).

Methods

HGF and HGK were exposed to eluates of Ceram X™, Filtek™ Silorane, Filtek™ Supreme XTE, Fusio™ Liquid Dentin and Vertise™ Flow. Eluates were created by storing material samples in respective cell culture medium, for 24 h and 72 h (n = 17), according to ISO 10993-12:2012. Cell response was evaluated at eluate exposure periods of 24 h and 72 h by (i) impedance analysis-based real-time monitoring of adhesion and proliferation, (ii) semi-quantitative indirect immunofluorescence (sq-IIF) detection of tissue-specific biomarkers, and (iii) ELISA-detection of pro-inflammatory interleukin (IL)-6.

Results

Generally, cell behavioural response towards the eluates was gradual in HGK and HGF, the latter exhibiting a less pronounced modulation per se. In HGK, ERK 1/2 was mainly activated after 24 h by Fusio™ Liquid Dentin and Vertise™ Flow, while an increase in biomarker expression occurred time-delayed. A 72 h exposure of HGK to eluates of Filtek™ Supreme XTE, Fusio™ Liquid Dentin and Vertise™ Flow significantly decreased secreted IL-6 amounts. In HGK, the impedance analysis revealed less proliferation and/or adhesion in case of Fusio™ Liquid Dentin and Vertise™ Flow with matched other composites.

Significance

In detail, protein expression and secretion is modulated particularly in terms of signal transduction, differentiation and inflammation. On cell biological level, all tested materials modulated the analysed features of cell behaviour with emphasis on the self-adhering composites.

中文翻译：

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牙龈细胞对牙科复合材料反应的调节

客观的

这项研究的目的是研究与临床相关的牙科复合材料在与暴露相关的靶细胞（即人牙龈成纤维细胞（HGF）和上皮角质形成细胞（HGK））中的细胞行为反应。

方法

将HGF和HGK暴露于Ceram X™，Filtek™Silorane，Filtek™Supreme XTE，Fusio™Liquid Dentin和Vertise™Flow的洗脱液中。根据ISO 10993-12：2012，通过将材料样本分别存储在各自的细胞培养基中24小时和72小时（n = 17）来创建洗脱液。通过（i）基于阻抗分析的实时粘附和增殖监测，（ii）组织特异性生物标记物的半定量间接免疫荧光（sq-IIF）检测，在24 h和72 h洗脱液暴露时间评估细胞反应和（iii）促炎性白介素（IL）-6的ELISA检测。

结果

通常，在HGK和HGF中，对洗脱液的细胞行为反应是逐渐的，后者本身表现出不太明显的调节作用。在HGK中，ERK 1/2主要在24小时后被Fusio™液体牙本质和Vertise™Flow激活，而生物标志物表达的增加是延时发生的。HGK暴露于Filtek™Supreme XTE，Fusio™液体牙本质和Vertise™Flow洗脱液中72小时会显着降低分泌的IL-6量。在HGK中，如果使用Fusio™Liquid Dentin和Vertise™Flow以及其他匹配的复合材料，则阻抗分析显示出较少的扩散和/或粘附。

意义

详细地，蛋白质表达和分泌特别是在信号转导，分化和炎症方面被调节。在细胞生物学水平上，所有测试材料都对细胞行为的分析特征进行了调节，重点是自粘复合材料。