Herein, a rapid, linker-free, single-step strategy for in situ synthesis of graphene quantum dot–luminol–Ag nanoparticle (GQD–luminol–AgNP) nanocomposites was designed by reducing AgNO₃ with an electrochemiluminescent reagent, luminol, in the presence of GQDs. The nanocomposites were characterized using transmission electron microscopy, UV-vis absorption spectroscopy, and EDS. The results indicated that many Ag nanoparticles (AgNPs) with an average diameter of about 5 nm were dispersed uniformly on the surface of GQD nanosheets. Luminol molecules were also decorated on the nanocomposite surface. Thus, the nanocomposites showed excellent electrochemiluminescence (ECL) activity in presence of H₂O₂. To build a glucose ECL-based biosensor, the prepared sensor was also modified with glucose oxidase (GOx). The GOx enzyme was covalently immobilized on the GQD–luminol–AgNP-modified glassy carbon electrode. The designed ECL biosensor showed great performance towards glucose detection in the concentration range between 25 and 250 μM with a satisfactory detection limit (8 μM).

中文翻译：

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原位修饰的纳米银颗粒修饰的石墨烯点 增强了鲁米诺的电化学发光，用于葡萄糖分析†

本文中，通过在存在下用电化学发光试剂鲁米诺还原AgNO ₃，设计了一种快速，无接头的单步策略，用于石墨烯量子点-鲁米诺-Ag纳米颗粒（GQD-鲁米诺-AgNP）纳米复合材料的原位合成。GQD。使用透射电子显微镜，紫外可见吸收光谱和EDS对纳米复合材料进行表征。结果表明，许多平均直径约为5 nm的Ag纳米颗粒（AgNPs）均匀地分散在GQD纳米片的表面上。鲁米诺分子也被修饰在纳米复合材料表面上。因此，在H ₂ O ₂存在下，纳米复合材料显示出优异的电化学发光（ECL）活性。。为了构建基于葡萄糖ECL的生物传感器，还使用葡萄糖氧化酶（GOx）修改了制备的传感器。GOx酶被共价固定在GQD-luminol-AgNP修饰的玻碳电极上。设计的ECL生物传感器在25至250μM的浓度范围内具有出色的葡萄糖检测性能，并具有令人满意的检测限（8μM）。