The combination of surface-enhanced Raman scattering (SERS) with high-throughput screening (HTS) has significant importance for highly sensitive and massive workload assays. Although fabrication of HTS–SERS substrates can be achieved by several methods, the high cost as well as large-equipment dependence limit their applications. Here, we report a simple method to fabricate HTS–SERS substrates within one-step process. The HTS–SERS substrate is fabricated by simply UV-irradiating a fluoroalkylsilane (FAS)-modified liquid-repellent TiO₂ surface in AgNO₃ solution through a photomask. Owing to the photocatalytic nature of TiO₂, the UV irradiation simultaneously triggers the degradation of the attached FAS and the generation of liquid-adhesive Ag nanoparticles (NPs) on exposed area. A HTS–SERS substrate could be directly obtained after UV irradiation. The deposited Ag NPs evidently enhance Raman signals, and the significant difference between the wettability of exposed area and masked area enables fast formation of high-throughput liquid droplet arrays through a simple dragging solution process. The fabrication method is applicable to various substrate materials, to introduce additional functionalities. The photocatalytic activity of TiO₂ also allows us to photobleach the residual analyte and Ag NPs after detection to recycle substrate. This single-step method is a highly promising candidate for the fabrication of HTS–SERS substrates.

中文翻译：

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高通量表面增强拉曼散射基板的单步制备

表面增强拉曼散射（SERS）与高通量筛选（HTS）的结合对于高度灵敏和大量工作量测定具有重要意义。尽管可以通过多种方法来制造HTS-SERS基板，但其高成本以及对设备的依赖性限制了它们的应用。在这里，我们报告了一种简单的方法，可以一步一步制造HTS-SERS基板。通过简单地通过光掩模在AgNO ₃溶液中对氟烷基硅烷（FAS）改性的疏液性TiO ₂表面进行紫外线照射，即可制成HTS-SERS基板。由于TiO ₂的光催化性质，紫外线照射同时会触发附着的FAS的降解，并在裸露的区域上产生液态粘合的Ag纳米颗粒（NPs）。紫外线照射后可以直接获得HTS-SERS底物。沉积的Ag NPs明显增强了拉曼信号，并且暴露区域和掩蔽区域的润湿性之间的显着差异使得可以通过简单的拖动溶液过程快速形成高通量液滴阵列。该制造方法适用于各种衬底材料，以引入附加的功能。TiO ₂的光催化活性还使我们能够在检测到残留底物和Ag NPs后进行光漂白，以回收底物。这种单步方法非常适合用于制造HTS-SERS基板。