To investigate the modulatory effect of oolong tea polyphenols (OTP) on intestinal microbiota, OTP was prepared by column chromatography and its influence on the gut flora structure was analyzed by high-throughput sequencing with a human flora-associated high fat diet (HFD) induced obesity mouse model. We observed a robust increase in bacterial biodiversity and the abundance of genera known to be butyrate- and acetate-producing bacteria. A large increase in Bacteroidetes with a decrease in Firmicutes was observed after the administration of OTP for 4 weeks, and the corresponding decrease in the Firmicutes/Bacteroidetes ratio reflected the positive modulatory effect of OTP on the intestinal microbiota. In addition, KEGG pathways for the biosynthesis of amino acids, carbon metabolism, and the ribosome were among the most differentially expressed genes after OTP intervention. The current study revealed that OTP rich in tea catechins, especially O-methylated derivatives, may have prebiotic-like activity and can be used as a functional food component with potential therapeutic utility to prevent obesity-related metabolic disorders by manipulating the intestinal microbiota.

中文翻译：

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用宏基因组学方法研究以乌龙茶多酚喂养的高脂饮食诱导的肥胖小鼠的肠道微生物组结构和功能†

为了研究乌龙茶多酚（OTP）对肠道菌群的调节作用，通过柱色谱法制备了OTP，并通过高通量测序，通过与人类菌群相关的高脂饮食（HFD）诱导了高通量测序，分析了其对肠道菌群结构的影响。肥胖小鼠模型。我们观察到细菌生物多样性的强劲增长以及已知是产生丁酸盐和乙酸盐的细菌的大量属。在给予OTP 4周后​​，观察到拟杆菌的数量大大增加，而纤毛虫的数量减少了，而纤毛虫/拟杆菌的数量相应减少了该比例反映了OTP对肠道菌群的正调节作用。此外，在OTP干预后，用于氨基酸的生物合成，碳代谢和核糖体的KEGG途径是表达最差的基因之一。当前的研究表明，富含茶儿茶素的OTP，特别是O-甲基化衍生物，可能具有类似益生元的活性，可以用作功能性食品成分，具有潜在的治疗作用，可通过控制肠道微生物群来预防肥胖相关的代谢紊乱。