This study is devoted on Allura red as food colorant preconcentration and determination in beverage, fruit juice and drink water samples is based on usage of Mn- and Cu- @ ZnS-NPs-AC as new sorbent for ultrasound-assisted-dispersive solid-phase microextraction (UA-DSPME) combined with ultraviolet-visible spectrophotometric based method (UV-Vis). Contribution of volume of eluent, pH, sorbent mass and sonication time on response following conduction of 28 experiments were optimized and investigated while their significantly justified according to p-value. Values of “Prob > F” less than 0.0500 is proportional with their significant influence on recovery of analyte. Under the optimum conditions 0.14 mL of THF pH of 2.5; 8 mg of sorbent and 3 min sonication time guide and help achievement of limit of quantification (LOQ) and limit of detection (LOD) of 6.08 and 20.26 ng mL^-1, respectively. The accuracy of method was validated according to calculation of recovery following spiking 400 and 600 ng mL^-1 to blank solution and recovery as more reliable indication of accuracy 93.41 and 102.17% recoveries with RSD <3.5%, which demonstrate the successful applicability of present method for real sample analysis. The maximum sorbent capacity was 50.0 mg g^-1 based on Langmuir isotherm as best model with high correlation coefficient. Combination of UA-DSPME and UV-Vis lead to higher sensitivity and lower cost for accurate and repeatable monitoring of Allura red level in beverage, Fruit juice and drink water samples with acceptable recovery and reasonable RSD%.

这项研究致力于将Allura红用作食品着色剂的浓缩和饮料，果汁和饮用水样品中的测定，其基础是使用Mn-和Cu- @ ZnS-NPs-AC作为超声辅助分散固相的新型吸附剂微萃取（UA-DSPME）与基于紫外可见分光光度法的方法（UV-Vis）相结合。优化并研究了洗脱液体积，pH，吸附剂质量和超声处理时间对进行28个实验后的响应的贡献，并根据p值显着证明了它们的合理性。小于0.0500的“ Prob> F”值与其对分析物回收率的显着影响成正比。在最佳条件下，0.14 mL THF的pH为2.5；^-1。根据在空白溶液中加标400和600 ng mL ^-1后回收率的计算结果，验证了方法的准确性，这是更可靠的指示准确度93.41和102.17％的回收率，RSD <3.5％，证明了本方法的成功适用性用于真实样品分析。基于Langmuir等温线作为具有高相关系数的最佳模型，最大吸附容量为50.0 mg g ^-1。UA-DSPME和UV-Vis的结合使用可提高灵敏度和成本，从而可准确，可重复地监测饮料，果汁和饮用水样品中的Allura红水平，回收率令人满意，RSD％合理。