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Fluorescent Silica Nanoparticles with Well-Separated Intensity Distributions from Batch Reactions
Nano Letters ( IF 10.8 ) Pub Date : 2018-01-02 00:00:00 , DOI: 10.1021/acs.nanolett.7b04978
Teresa Kao 1 , Ferdinand Kohle 1, 2 , Kai Ma 1 , Tangi Aubert 1, 3 , Alexander Andrievsky 1, 2 , Ulrich Wiesner 1
Affiliation  

Silica chemistry provides pathways to uniquely tunable nanoparticle platforms for biological imaging. It has been a long-standing problem to synthesize fluorescent silica nanoparticles (SNPs) in batch reactions with high and low fluorescence intensity levels for reliable use as an intensity barcode, which would greatly increase the number of molecular species that could be tagged intracellularly and simultaneously observed in conventional fluorescence microscopy. Here, employing an amino-acid catalyzed growth, highly fluorescent SNP probes were synthesized with sizes <40 nm and well-separated intensity distributions, as mapped by single-particle imaging techniques. A seeded growth approach was used to minimize the rate of secondary particle formation. Organic fluorescent dye affinity for the SNP during shell growth was tuned using specifics of the organosilane linker chemistry. This work highlights design considerations in the development of fluorescent probes with well-separated intensity distributions synthesized in batch reactions for single-particle imaging and sensing applications, where heterogeneities across the nanoparticle ensemble are critical factors in probe performance.

中文翻译:

批处理反应的强度分布均匀的荧光二氧化硅纳米粒子

二氧化硅化学为生物成像提供了通往独特可调纳米颗粒平台的途径。在具有高和低荧光强度水平的批反应中合成荧光二氧化硅纳米颗粒(SNP)以可靠地用作强度条形码是一个长期存在的问题,这将大大增加可在细胞内同时标记的分子种类的数量在常规荧光显微镜下观察到的。在这里,利用氨基酸催化的生长,合成了高度荧光的SNP探针,尺寸小于40 nm,并且强度分布良好,如单粒子成像技术所示。使用种子生长方法来最小化次级颗粒形成的速率。壳生长过程中有机荧光染料对SNP的亲和力可通过有机硅烷接头化学的细节进行调节。这项工作突出了在荧光探针的开发中的设计考虑因素,该荧光探针具有在批反应中合成的强度分布良好的单分子成像和传感应用,其中纳米粒子整体的异质性是探针性能的关键因素。
更新日期:2018-01-02
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