A new polymer flow-cell for chemiluminescence detection (CLD) has been designed and developed by diverging multiple linear channels from a common centre port in a radial arrangement. The fabrication of radial flow-cell by 3D PolyJet printing and fused deposition modeling (FDM) has been evaluated, and compared with a similarly prepared spiral flow-cell design commonly used in chemiluminescence detectors. The radial flow-cell required only 10 h of post-PolyJet print processing time as compared to ca. 360 h long post-PolyJet print processing time required for the spiral flow-cell. Using flow injection analysis, the PolyJet 3D printed radial flow-cell provided an increase in both the signal magnitude and duration, with an average increase in the peak height of 63% and 58%, peak area of 89% and 90%, and peak base width of 41% and 42%, as compared to a coiled-tubing spiral flow-cell and the PolyJet 3D printed spiral flow-cell, respectively. Computational fluid dynamic (CFD) simulations were applied to understand the origin of the higher CLD signal obtained with the radial flow-cell design, indicating higher spatial coverage near the inlet and lower linear velocities in the radial flow-cell. The developed PolyJet 3D printed radial flow-cell was applied in a new ion chromatography chemiluminescence based assay for the detection of H2O2 in urine and coffee extracts.

中文翻译：

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用于化学发光检测的新型 3D 打印径向流通池：在离子色谱法测定尿液和咖啡提取物中的过氧化氢中的应用

一种用于化学发光检测 (CLD) 的新型聚合物流通池已被设计和开发，方法是将多个线性通道从一个公共中心端口以径向排列方式分叉。已经评估了通过 3D PolyJet 打印和熔融沉积建模 (FDM) 制造径向流通池，并与化学发光检测器中常用的类似制备的螺旋流通池设计进行了比较。与大约 10 小时相比，径向流通池仅需要 10 小时的 PolyJet 后打印处理时间。螺旋流通池需要 360 小时长的 PolyJet 打印后处理时间。使用流动注射分析，PolyJet 3D 打印径向流动池增加了信号幅度和持续时间，峰高平均增加了 63% 和 58%，峰面积增加了 89% 和 90%，峰值基础宽度为 41% 和 42%，分别与连续管螺旋流通池和 PolyJet 3D 打印螺旋流通池相比。应用计算流体动力学 (CFD) 模拟来了解通过径向流通池设计获得的较高 CLD 信号的起源，表明入口附近的空间覆盖率更高，径向流通池中的线速度较低。开发的 PolyJet 3D 打印径向流通池被应用于一种新的基于离子色谱化学发光的检测方法，用于检测尿液和咖啡提取物中的 H2O2。表明入口附近的空间覆盖率较高，径向流通池中的线速度较低。开发的 PolyJet 3D 打印径向流通池被应用于一种新的基于离子色谱化学发光的检测方法，用于检测尿液和咖啡提取物中的 H2O2。表明入口附近的空间覆盖率较高，径向流通池中的线速度较低。开发的 PolyJet 3D 打印径向流通池被应用于一种新的基于离子色谱化学发光的检测方法，用于检测尿液和咖啡提取物中的 H2O2。