Abstract Detecting pork contamination is critical for halal food preparation. A fragment of species-specific DNA from swine mitochondrial genome was selected as a target, and two oligonucleotides complementary to the target were used as probes for pork identification. The probe-conjugated gold nanoparticles bound to the target DNA, leading to the aggregation of gold nanoparticles and thus causing the colour of the gold nanoparticles to change from red to blue. More than 0.1 μmol/L of DNA led to an obvious colour change, which was observed by naked eyes after dotting test solutions onto a thin layer chromatography (TLC) plate. Furthermore, 0.001–1 μmol/L of the target was semi-quantified by using a digital camera and image processing software. This colourimetric detection might be a promising technique for differentiation of pork from other animal meat products without using any sophisticated machines, and thus will be used for halal authentication.

中文翻译：

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使用金纳米粒子比色检测用于清真认证的猪特异性 DNA

摘要 检测猪肉污染对于清真食品制备至关重要。选择猪线粒体基因组的物种特异性DNA片段作为靶标，并使用与靶标互补的两条寡核苷酸作为猪肉鉴定的探针。探针结合的金纳米粒子与目标 DNA 结合，导致金纳米粒子聚集，从而导致金纳米粒子的颜色从红色变为蓝色。超过 0.1 μmol/L 的 DNA 会导致明显的颜色变化，这是在薄层色谱 (TLC) 板上点试溶液后肉眼观察到的。此外，使用数码相机和图像处理软件对 0.001–1 μmol/L 的目标进行了半定量。