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Non-targeted investigation of benthic invertebrates (Chironomus riparius) exposed to wastewater treatment plant effluents using nanoliquid chromatography coupled to high-resolution mass spectrometry
Chemosphere ( IF 8.8 ) Pub Date : 2018-01-02 , DOI: 10.1016/j.chemosphere.2018.01.001
Alexandra Berlioz-Barbier , Audrey Buleté , Aurélie Fildier , Jeanne Garric , Emmanuelle Vulliet

Nanoliquid chromatography (nanoLC) was coupled to high-resolution mass spectrometry (HRMS) to perform a non-targeted investigation on benthic invertebrates, Chironomus riparius exposed to wastewater treatment plant (WWTP) effluents. Insect larvae represent a complex and low-weight matrix that required the use of a miniaturized Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method of extraction followed by nanoLC-HRMS to perform the analysis. The optimization of this coupling in terms of separation conditions including trapping step, detection conditions and data treatment provided reproducible fingerprints on insect larvae exposed to WWTP effluents with both in situ and ex-situ approaches. Statistical treatments such as principal component analysis highlighted the impact of WWTP effluents on the metabolome of insect larvae and showed the influence of exposure conditions. The identification of discriminating signals (m/z, tR) matched with several potential endogenous biomarkers. These are mainly fatty acids, indicating a change in lipid metabolism that can be correlated with exposure to WWTP effluents. Several xenobiotics have also been detected, including ibuprofen and propranolol, whose identities have been confirmed by analytical standards. This work demonstrates the effectiveness and sensitivity of nanoLC-HRMS based environmental non-targeted approaches in ecotoxicological studies and provides the first profiling data for a very small aquatic invertebrate.



中文翻译:

使用纳米液相色谱-高分辨率质谱法对暴露于废水处理厂废水中的底栖无脊椎动物(Chironomus riparius)进行非目标研究

纳米液相色谱法(nanoLC)与高分辨率质谱法(HRMS)结合使用,对暴露于废水处理厂(WWTP)废水的底栖无脊椎动物无脊椎动物Chironomus riparius进行了非目标研究。昆虫幼虫代表一个复杂且重量轻的基质,需要使用微型化的快速,简便,廉价,有效,坚固耐用且安全的提取(QuEChERS)方法,然后使用nanoLC-HRMS进行分析。这种耦合的中的分离条件的条件,包括捕获步骤,检测条件和数据处理设置在暴露于污水处理厂的流出物与两个昆虫幼虫可再现的指纹优化原位易地方法。统计处理(例如主成分分析)突出显示了污水处理厂废水对昆虫幼虫代谢组的影响,并显示了暴露条件的影响。识别鉴别信号(m / z,t R)与几种潜在的内源性生物标记物相匹配。这些主要是脂肪酸,表明脂质代谢的变化可能与暴露于污水处理厂废水有关。还检测了几种异生素,包括布洛芬和普萘洛尔,其身份已通过分析标准确认。这项工作证明了基于nanoLC-HRMS的环境非靶向方法在生态毒理学研究中的有效性和敏感性,并为非常小的水生无脊椎动物提供了第一个分析数据。

更新日期:2018-01-02
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