This study deals with synthesis of a new set of benzofuran and 5H-furo[3,2-g]chromone linked various heterocyclic functionalities using concise synthetic approaches aiming to gain new antiproliferative candidates against MCF-7 breast cancer cells of p38α MAP kinase inhibiting activity. The biological data proved the significant sensitivity of breast cancer cell lines MCF-7 towards most of the prepared compounds in comparison with doxorubicin. In addition, compounds IIa,b, Va,b, VIa,b, VIIa,b, VIIIa,b, XIc showed significant in vitro p38α MAPK inhibiting potency comparable to the reference standard SB203580. Cell cycle analysis and apoptosis detection data demonstrated that compound VIa induced G2/M phase arrest and apoptosis in MCF-7 cancer cells, in addition to its activation of the caspases-9 and -3. Gold molecular docking studies rationalized the highly acceptable correlation between the calculated docking scores of fitness and the biological data of p38α MAP kinase inhibition. The newly prepared benzofuran and 5H-furo[3,2-g]chromone derivatives might be considered as new promising nuclei in anti-breast cancer chemotherapeutics for further functionalization, optimization and in-depth biological studies.

这项研究涉及使用简洁的合成方法合成一组新的苯并呋喃和5 H-呋喃并[3,2- g ]色酮连接的各种杂环功能，旨在获得针对p38αMAP激酶抑制作用的MCF-7乳腺癌细胞的新抗增殖候选物。活动。生物学数据证明，与阿霉素相比，乳腺癌细胞系MCF-7对大多数制备的化合物具有显着的敏感性。另外，化合物IIa，b，Va，b，VIa，b，VIIa，b，VIIIa，b，XIc在体外显示出显着的p38αMAPK抑制效能可与参考标准SB203580媲美。细胞周期分析和细胞凋亡检测数据表明，化合物VIa除了激活caspases-9和-3之外，还诱导MCF-7癌细胞的G2 / M期阻滞和细胞凋亡。金的分子对接研究合理化了计算的对接分数和p38αMAP激酶抑制生物学数据之间高度可接受的相关性。新制备的苯并呋喃和5 H-呋喃并[3,2- g ]色酮衍生物可能被认为是抗乳腺癌化学治疗中新的有希望的核，用于进一步的功能化，优化和深入的生物学研究。