Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to protein adsorption. Short-chain ethylene oxide (EO) oligomers have been shown to improve the protein resistance of planar Au surfaces. We describe the application of these EO oligomers for improved protein resistance of 30 nm spherical gold nanoparticles (AuNPs). Functionalized AuNPs were characterized using UV-Vis spectroscopy, dynamic light scattering (DLS), and zeta potential measurements. Capillary electrophoresis (CE) was used for separation and quantitation of AuNPs and AuNP-protein mixtures. Specifically, nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) was employed for the determination of equilibrium and rate constants for binding between citrate-stabilized AuNPs and two model proteins, lysozyme and fibrinogen. Semi-quantitative CE analysis was carried out for mixtures of EO-functionalized AuNPs and proteins, and results demonstrated a 2.5-fold to 10-fold increase in protein binding resistance to lysozyme depending on the AuNP surface functionalization and a 15-fold increase in protein binding resistance to fibrinogen for both EO oligomers examined in this study.

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在纳米材料表面形成的蛋白质电晕在纳米材料的生物利用度和细胞吸收中起着重要作用。用低聚乙二醇（OEG）修饰表面是提高纳米材料对蛋白质吸附的抵抗力的常用方法。短链环氧乙烷（EO）低聚物已显示可改善平面Au表面的蛋白抗性。我们描述了这些EO低聚物在30 nm球形金纳米颗粒（AuNPs）改善蛋白抗性中的应用。使用UV-Vis光谱，动态光散射（DLS）和Zeta电位测量对功能化的AuNP进行了表征。毛细管电泳（CE）用于分离和定量AuNPs和AuNP蛋白混合物。具体来说，平衡混合物的非平衡毛细管电泳（NECEEM）用于确定柠檬酸盐稳定的AuNP与两种模型蛋白（溶菌酶和纤维蛋白原）结合的平衡和速率常数。对EO功能化的AuNP和蛋白质的混合物进行了半定量CE分析，结果表明取决于AuNP表面功能化的蛋白质与溶菌酶的结合力增加了2.5到10倍，蛋白质增加了15倍在这项研究中检查的两个EO低聚物对纤维蛋白原的结合抗性。

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