The development of a cell-growth substrate that provides a nature-like microenvironment, promotes cell adhesion, and maintains the cells’ functional activities is a research focus in the field of tissue engineering. In the present study, three-dimensional micro-nano multiscale fiber-based substrates were developed by depositing biocompatible polycaprolactone (PCL)/PCL-Chitosan (C)/PCL-C-Gelatin (G) electrospun nanofibers (NFs) on the outer surface of hollow fiber membranes (HFMs) in one step. A comparison study with regard to physico-chemical characterization, hemocompatibility, cytotoxicity, and cellular functionality was performed with the developed matrices. The PCL-C-G NFs-deposited HFMs-based matrix showed superior hemocompatibility for blood-contact applications. The cytotoxicity of these matrices was found to be minimal. HepG2 cells exhibited an exceptionally robust adherence and proliferated growth on the matrix with the formation of characteristic multi-cellular spheroids. Furthermore, cell functional activities such as albumin secretion, urea synthesis, and cytochrome P450 specific activity were measured for the developed matrices. The developed three-dimensional multiscale fibers-based matrix can be a potential membrane for bioreactor and bio-artificial liver applications.

中文翻译：

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三维多尺度纤维基质：为生物人工肝应用增加HepG2功能维持能力的开发和表征

提供一种类似于自然环境的微环境，促进细胞粘附并维持细胞功能活性的细胞生长基质的开发是组织工程领域的研究重点。在本研究中，通过在外表面沉积生物相容性聚己内酯（PCL）/ PCL-壳聚糖（C）/ PCL-C-明胶（G）电纺纳米纤维（NFs），开发了基于三维微纳米多尺度纤维的基底一步即可完成中空纤维膜（HFM）的制造。用已开发的基质进行了有关理化特性，血液相容性，细胞毒性和细胞功能的比较研究。PCL-CG NFs沉积的基于HFMs的基质在血液接触应用中显示出优异的血液相容性。发现这些基质的细胞毒性是最小的。HepG2细胞在基质上表现出异常强大的粘附力和增殖性生长，形成了特征性的多细胞球体。此外，对于已开发的基质，还测量了细胞功能活性，例如白蛋白分泌，尿素合成和细胞色素P450的比活性。所开发的基于三维多尺度纤维的基质可以成为生物反应器和生物人工肝应用的潜在膜。