Through the use of stem cell-derived cardiac myocytes, tissue-engineered human myocardial constructs are poised for modeling normal and diseased physiology of the heart, as well as discovery of novel drugs and therapeutic targets in a human relevant manner. This review highlights the recent bioengineering efforts to recapitulate microenvironmental cues to further the maturation state of newly differentiated cardiac myocytes. These techniques include long-term culture, co-culture, exposure to mechanical stimuli, 3D culture, cell-matrix interactions, and electrical stimulation. Each of these methods has produced various degrees of maturation; however, a standardized measure for cardiomyocyte maturation is not yet widely accepted by the scientific community.

中文翻译：

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工程化的干细胞衍生心肌细胞成熟的微环境

通过使用干细胞衍生的心肌细胞，组织工程化的人类心肌构建体有望用于对心脏的正常和患病生理进行建模，并以与人类相关的方式发现新药和治疗靶标。这项审查突出了最近的生物工程工作，以概括微环境的线索，以进一步促进新分化的心肌细胞的成熟状态。这些技术包括长期培养，共培养，暴露于机械刺激，3D培养，细胞-基质相互作用和电刺激。这些方法中的每一种都产生了不同程度的成熟度。然而，用于心肌细胞成熟的标准化措施尚未被科学界广泛接受。