Potato native and wound healing periderms contain an external multilayered phellem tissue (potato skin) consisting of dead cells whose cell walls are impregnated with suberin polymers. The phellem provides physical and chemical barriers to tuber dehydration, heat transfer, and pathogenic infection. Previous RNAi-mediated gene silencing studies in native periderm have demonstrated a role for a feruloyl transferase (FHT) in suberin biosynthesis and revealed how its down-regulation affects both chemical composition and physiology. To complement these prior analyses and to investigate the impact of FHT deficiency in wound periderms, a bottom-up methodology has been used to analyze soluble tissue extracts and solid polymers concurrently. Multivariate statistical analysis of LC-MS and GC-MS data, augmented by solid-state NMR and thioacidolysis, yields two types of new insights: the chemical compounds responsible for contrasting metabolic profiles of native and wound periderms, and the impact of FHT deficiency in each of these plant tissues. In the current report, we confirm a role for FHT in developing wound periderm and highlight its distinctive features as compared to the corresponding native potato periderm.

中文翻译：

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马铃薯原生和伤口周皮受到 FHT 下调的不同影响，FHT 是一种木栓素阿魏酰转移酶

马铃薯原生和伤口愈合外皮包含外部多层木栓组织（马铃薯皮），由死细胞组成，其细胞壁浸渍有木栓质聚合物。木栓为块茎脱水、传热和病原体感染提供物理和化学屏障。先前在天然周皮中进行的 RNAi 介导的基因沉默研究已经证明了阿魏酰转移酶 (FHT) 在木栓质生物合成中的作用，并揭示了其下调如何影响化学成分和生理学。为了补充这些先前的分析并研究 FHT 缺乏对伤口周皮的影响，自下而上的方法已被用于同时分析可溶性组织提取物和固体聚合物。LC-MS 和 GC-MS 数据的多变量统计分析，通过固态核磁共振和硫代酸解增强，产生了两种类型的新见解：负责对比天然和伤口周皮代谢特征的化合物，以及 FHT 缺乏对这些植物组织中的每一个的影响。在当前的报告中，我们确认了 FHT 在开发伤口周皮中的作用，并突出了其与相应的天然马铃薯周皮相比的独特特征。