Fundamental advances in the field of biomolecular engineering present researchers with the unprecedented opportunity to apply molecular design strategies to modify naturally occurring proteins and generate new molecules for targeted disease therapy. In particular, the development of immunotherapeutics such as cytokine-based and antibody-based drugs has been empowered by evolving technologies and insights from protein engineering. In this review, we highlight specific examples that demonstrate the growing influence of biomolecular engineering on the discovery and design of immunotherapeutics, with particular emphasis on three strategies in biologics development: (1) Cytokine engineering, (2) Cytokine-targeted antibody design, and (3) Fine-tuning the disease-targeting mechanisms of antibody drugs. These strategies have already yielded significant success in the clinic and promise to continue informing and improving the drug development landscape.

生物分子工程领域的根本进步为研究人员提供了前所未有的机会，可以应用分子设计策略来修饰天然存在的蛋白质并产生用于靶向疾病治疗的新分子。尤其是，免疫疗法的发展，例如基于细胞因子和基于抗体的药物的发展，都受到了蛋白质工程学不断发展的技术和见解的推动。在这篇综述中，我们重点介绍一些具体的例子，这些例子证明了生物分子工程学对免疫治疗剂的发现和设计的影响力日益增强，尤其着重于生物制剂开发中的三种策略：（1）细胞因子工程学，（2）细胞因子靶向抗体设计，以及（3）微调抗体药物的疾病靶向机制。