Bispyribac sodium (BS), is a selective, systemic and post emergent herbicide used to eradicate grasses and broad leaf weeds. Extensive use of this herbicide has engendered serious environmental concerns. Hence it is important to develop strategies for bioremediation of BS in a cost effective and environment friendly way. In this study a bacterial consortium named BDAM, comprising three novel isolates Achromobacter xylosoxidans (BD1), Achromobacter pulmonis (BA2), and Ochrobactrum intermedium (BM2), was developed by virtue of its potential for degradation of BS. Different culture conditions (temperature, pH and inoculum size) were optimized for degradation of BS by the consortium BDAM and the mutual interactions of these parameters were analysed using a 2³ full factorial central composite design (CCD) based on Response Surface Methodology (RSM). The optimal values for temperature, pH and inoculum size were found to be 40 °C, 8 and 0.4 g/L respectively to achieve maximum degradation of BS (85.6%). Moreover, the interactive effects of these parameters were investigated using three dimensional surface plots in terms of maximum fitness function. Importantly, it was concluded that the newly developed consortium is a potential candidate for biodegradation of BS in a safe, cost-effective and environmentally friendly manner.

Bispyribac钠（BS）是一种选择性的，内吸的和出苗后的除草剂，用于根除草和阔叶杂草。广泛使用这种除草剂引起了严重的环境问题。因此，以经济有效且环境友好的方式开发BS生物修复策略非常重要。在这项研究中，由于其具有降解BS的潜力，因此开发了一个名为BDAM的细菌协会，该协会包括三种新型分离物木糖氧化无色杆菌（BD1），肺部无色杆菌（BA2）和中间O骨O虫（BM2）。针对不同的培养条件（温度，pH和接种量），通过BDAM联盟对BS的降解进行了优化，并使用2分析了这些参数的相互作用³基于响应表面方法论（RSM）的全因子中央复合设计（CCD）。发现温度，pH和接种物大小的最佳值分别为40°C，8和0.4 g / L，以实现BS的最大降解（85.6％）。此外，在最大适应度函数方面，使用三维曲面图研究了这些参数的交互作用。重要的是，得出的结论是，新成立的财团以安全，经济高效和环境友好的方式可能成为BS生物降解的潜在候选者。