Human norovirus (HuNoV) is a major foodborne virus causing gastroenteritis outbreaks in humans. Salad products can be vectors of transmission for foodborne viruses such as HuNoV when these products are contaminated naturally or through unsanitary food handling. Therefore, development of simple, reliable and sensitive techniques for the detection of HuNoV in salad products is needed to ensure food safety. The purpose of our study was to optimize a method for the detection of HuNoV in artificially contaminated salad products. To this end, 2 different kinds of salads (fruit salads and vegetable salads) were experimentally inoculated with HuNoV GI, HuNoV GII, and MS2 suspensions. The selected method was based on treatment with pectinase followed by Trizol-chloroform purification, and the recovery efficiencies were 6.07% to 26.52% for HuNoV GI and 5.54% to 37.36% for HuNoV GII. MS2 was used as the process control, and the recovery efficiencies for fruit salad and vegetable salad samples were 38.57% and 41.13%, respectively. The optimized method could be applied in diagnostic laboratories to identify NoV contamination in composite foods, such as salad products, should an event of foodborne outbreak occur.

中文翻译：

---

检测水果和蔬菜沙拉中 GI 和 GII 诺如病毒的提取方法的评价

人类诺如病毒 (HuNoV) 是一种主要的食源性病毒，可导致人类胃肠炎暴发。当这些产品被自然污染或通过不卫生的食品处理受到污染时，沙拉产品可能成为 HuNoV 等食源性病毒的传播载体。因此，需要开发简单、可靠和灵敏的技术来检测沙拉产品中的 HuNoV，以确保食品安全。我们研究的目的是优化检测人工污染沙拉产品中 HuNoV 的方法。为此，用 HuNoV GI、HuNoV GII 和 MS2 悬浮液对 2 种不同的沙拉（水果沙拉和蔬菜沙拉）进行了实验接种。所选方法基于果胶酶处理，然后 Trizol-氯仿纯化，HuNoV GI 和 5 的回收效率为 6.07% 至 26.52%。HuNoV GII 为 54% 至 37.36%。MS2 作为过程控制，水果沙拉和蔬菜沙拉样品的回收率分别为 38.57% 和 41.13%。如果发生食源性爆发事件，优化的方法可用于诊断实验室，以识别复合食品（如沙拉产品）中的 NoV 污染。