A liquid chromatography quadruple time-of-flight mass spectrometry (LC–QqTOF-MS/MS) method was developed for simultaneous quantitative analysis of ibuprofen (IBU), 1- and 2-hydroxyibuprofen (1-OH IBU and 2-OH IBU), and carboxyibuprofen (CBX IBU) while preserving the ability of the instrument to get precursor and product ion mass spectra of non-target compounds. The trigger was the precursor ions reaching 100 cps intensity. Sample preparation was carried out by ultrasound solid-liquid extraction with methanol as extraction solvent at pH < 2 followed by solid-phase extraction (SPE) clean-up using STRATA-X cartridges and methanol as an eluent. Linearity was obtained in the range 50–10,000 ng mL⁻¹ for IBU, each OH IBU and CBX IBU (r ≥ 0.99). The proposed method was satisfactorily validated showing absolute recoveries of > 70% for all target analytes at low and high concentration levels. The lowest limit of quantification was < 50 ng g⁻¹ in plant. This method was applied to investigate IBP behavior in cowpea (Vigna unguiculata (L.) Walp) treated at high IBU concentrations and its presence in vegetables irrigated with treated water. Up to 46 metabolites, mostly hydroxylated metabolites and conjugates with hexosides and amino acids, were identified. The most abundant metabolites were also identified in an eggplant sample.

中文翻译：

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使用液相色谱-四极杆飞行时间质谱分析豆（Vigna unguiculata L. Walp）的根，茎和种子中的布洛芬及其主要代谢物：吸收，代谢和易位

建立了液相色谱四倍飞行时间质谱（LC-QqTOF-MS / MS）方法，用于同时定量分析布洛芬（IBU），1-和2-羟基布洛芬（1-OH IBU和2-OH IBU）的方法，以及羧基布洛芬（CBX IBU），同时保留了仪器获得非目标化合物的前驱物和产物离子质谱的能力。触发因素是前体离子达到100 cps的强度。样品制备是通过在pH <2的条件下用甲醇作为萃取溶剂进行超声固液萃取，然后使用STRATA-X柱和甲醇作为洗脱液进行固相萃取（SPE）净化。线性度的范围内获得50-10,000每mL ^-1为IBU，每个OH IBU和CBX IBU（ř ≥0.99）。令人满意地验证了所提出的方法，该方法在低浓度和高浓度下对所有目标分析物的绝对回收率均> 70％。植物的最低定量限为<50 ng g ^-1。该方法用于调查以高IBU浓度处理的cow豆（Vigna unguiculata（L.）Walp）的IBP行为及其在用处理过的水灌溉的蔬菜中的存在。鉴定了多达46种代谢物，主要是羟基化代谢物以及与己糖苷和氨基酸的结合物。在茄子样品中也鉴定出了最丰富的代谢产物。