Achyranthes bidentata is an important Traditional Chinese Medicine for the treatment of osteoporosis. In this study, A. bidentata polysaccharide (ABPB), which was extracted with alkali from the root of A. bidentata at room temperature, significantly increased the bone mineral density, bone mineral content, trabecular thickness, trabecular number and biomechanical properties of ovariectomized (OVX) rats, indicating that ABPB had prominent curative effects on osteoporosis in OVX rats. A novel polysaccharide (ABPB-3) was purified from ABPB, and its structure was characterized as a repeating unit consisting of →4)-α-D-GalpA-(1→, →2,4)-α-L-Rhap-(1→, →5)-α-L-Araf-(1→, →2,3,5)-α-L-Araf-(1→, →3)-β-D-Galp-(1→, →3,4,6)-β-D-Galp-(1→, terminated with α-L-Araf, α-L-Rhap and β-D-Galp. Up to now, there were no literature reports relevant to the structure of ABPB-3. In the zebrafish model of glucocorticoid-induced osteoporosis (GIOP), ABPB-3 significantly increased the relative fluorescence intensity of the skull bone mass in a concentration-dependent manner, indicating that it stimulated bone formation activity. Thus, ABPB and ABPB-3 have the potential to be used for the anti-osteoporosis medicine.

牛膝是治疗骨质疏松症的重要中药。在这项研究中，牛膝多糖（ABPB），将其与从根碱提取牛膝在室温下，显著增加骨密度，骨矿物质含量，骨小梁厚度，小梁数目和卵巢切除的生物力学特性（ OVX）大鼠，表明ABPB对OVX大鼠的骨质疏松症具有显着的治愈作用。一种新型多糖（ABPB-3）从ABPB纯化，其结构的特征在于作为由→4）的重复单元- α -D-半乳糖p A-（1→，→2,4） - α -L- Rha p-（1→，→5）-α-L-阿糖胞苷˚F - （1→，→2,3,5） - α -L-阿糖胞苷˚F - （1→，→3） - β -D-Gal的p - （1→，→3,4,6- ） - β -D-Gal的p - （1→，具有封端的α -L-阿糖胞苷˚F，α -L-鼠李p和β -D-半乳糖p。到目前为止，还没有关于ABPB-3结构的文献报道。在糖皮质激素诱发的骨质疏松症（GIOP）的斑马鱼模型中，ABPB-3以浓度依赖的方式显着增加了颅骨质量的相对荧光强度，表明它刺激了骨形成活性。因此，ABPB和ABPB-3具有用于抗骨质疏松症药物的潜力。