We have previously reported that the keratan sulfate (KS) disaccharide was branched to the C-3 position of glucuronate in chondroitin sulfate (CS)-E derived from the Mactra chinensis. We carried out the comprehensive disaccharide analysis of GAGs from 10 shellfish, Ruditapes philippinarum, Scapharca broughtonii, Mizuhopecten yessoensis, Turbo cornutus, Crassostrea nippona, Corbicula japonica, Mytilus galloprovincialis, Neptunea intersculpta, Pseudocardium sachalinense and Crassostrea gigas, to better understand the glycan structures in marine organisms. The contents of CS, heparan sulfate and hyaluronic acid and their compositions depend on the species of shellfish. Interestingly, a peak corresponding to a pentasaccharide containing KS disaccharide was observed when GAGs from T. cornutus was treated with chondroitinase (Chase) ACII but not Chase ABC. In addition, unidentified peaks were also observed when CS derived from R. philippinarum, S. broughtonii were treated with Chase ACII. These results suggest the presence of additional unidentified structure of CS in these shellfish.

我们先前已报道，硫酸角质素（KS）的二糖被分支到葡糖醛酸的硫酸软骨素（CS）-E的C-3位置从派生四角羊草。我们从10贝类，进行GAG的综合分析，双糖菲律宾蛤仔，毛蚶broughtonii，Mizuhopecten虾夷扇贝，角蝾螺，牡蛎nippona，蚬粳稻，紫贻贝，Neptunea intersculpta，Pseudocardium sachalinense和太平洋牡蛎，以更好地了解海洋生物中的聚糖结构。CS，硫酸乙酰肝素和透明质酸的含量及其组成取决于贝类的种类。有趣的是，当用软骨素酶（Chase）ACII而不是Chase ABC处理来自角皮癣菌的GAG时，观察到对应于含有KS二糖的五糖的峰。此外，当用Chase ACII处理得自菲律宾蛤仔，布氏链霉菌的CS时，也观察到未鉴定的峰。这些结果表明在这些贝类中还存在CS的其他未知结构。