Grape canes, the residues from the annual pruning of vines, contain high levels of inducible (E)-resveratrol and also oligomeric stilbenoids and proanthocyanidins. These two families of phenolic compounds are bioactive, but to quantify them in a single chromatographic run using only ultraviolet detection is a difficult task. To overcome this limitation, a chromatographic method was developed using a core shell column for separation, an ultraviolet-visible diode array detector (DAD) and a fluorescence (FL) detector connected in series for quantification, with an electrospray ionization interface (ESI) and a triple quadrupole mass spectrometric detector (MS/MS) added for identification of the analytes. The proanthocyanidins (+)-catechin, (−)-epicatechin, procyanidins B1, B2, and C1, an unknown dimer and trimer, two prodelphinidin dimers, and monogallate procyanidin dimers were detected in the tested grape cane samples. The stilbenoids detected were (E)-resveratrol, (E)-piceatannol, (E)-piceid, (E)-ε-viniferin, vitisin B, a glycosylated monomer, three oxidized dimers, an unknown dimer and a tetramer, pallidol, hopeaphenol, (E)-δ-viniferin, and (E)-ω-viniferin. However, this method required 60 min for each analysis. A faster and more efficient method for quantitative analysis was developed based on HPLC-DAD-FL, reducing the time required to 24 min for the simultaneous quantification of proanthocyanidins and stilbenoids in Cabernet Sauvignon, Pinot Noir, and Tintorera grape canes stored at controlled temperatures and relativity humidities for 134 days after pruning. To the best of our knowledge, this is the first time a prodelphinidin dimer has been quantified in grape canes. The incorporation of fluorescence detection in series with DAD not only allowed the quantification of proanthocyanidins, it also improved the detectability of some minor stilbenoids present in the canes, such as (E)-piceid. The (E)-resveratrol and (E)-piceatannol levels increased significantly during cane storage, while those of (E)-ε-viniferin and ampelopsin A did not show significant increases. The relative humidity had a determining effect on the levels of (E)-resveratrol and (E)-piceatannol in the canes of all varieties studied; their concentrations were higher at a relative humidity of 60% than at 70%. This is the first time that the proanthocyanidin profiles of canes stored after pruning were monitored under controlled conditions of temperature, time and relative humidity. The concentration of (−)-epicatechin decreased during storage under both relative humidities. Furthermore, the levels of proanthocyanidin B1 and the prodelphinidin dimer also decreased to a certain extent.

葡萄藤是每年修剪葡萄藤的残留物，含有高水平的可诱导性（E）-白藜芦醇，以及低聚类芪和原花青素。这两个家族的酚类化合物具有生物活性，但是仅使用紫外线检测在单个色谱图中对它们进行定量是一项艰巨的任务。为克服此限制，开发了一种色谱方法，该方法使用核壳柱进行分离，将紫外可见二极管阵列检测器（DAD）和荧光（FL）检测器串联连接进行定量，并采用电喷雾电离接口（ESI）和添加了三重四极杆质谱检测器（MS / MS），用于鉴定分析物。在测试的甘蔗样品中检测到原花青素（+）-儿茶素，（-）-表儿茶素，原花青素B1，B2和C1，未知的二聚体和三聚体，两个原花青素二聚体和单没食子酸酯原花青素二聚体。E）-白藜芦醇，（E）-皮三醇，（E）-哌啶，（E）-ε-viniferin，葡萄球蛋白B，糖基化单体，三个氧化二聚体，一个未知二聚体和一个四聚体，pallidol，希望酚，（E） -δ-viniferin和（E）-ω-viniferin。但是，此方法每次分析需要60分钟。开发了一种基于HPLC-DAD-FL的更快，更高效的定量分析方法，将同时定量在控制温度和温度下保存的赤霞珠，黑比诺和丁托拉葡萄藤中原花色素和类胡萝卜素所需的时间减少到24分钟。修剪后134天保持相对湿度。据我们所知，这是葡萄藤中首次对原前啡肽二聚体进行定量。与DAD串联的荧光检测的结合不仅允许原花青素的定量，而且还提高了甘蔗中存在的一些次要类胡萝卜素的可检测性，例如（E）-piceid。（E）-白藜芦醇和（E）-皮香酚水平在甘蔗贮藏期间显着增加，而（E）-ε-葡萄素和两性霉素A的水平未显示出显着增加。相对湿度对所有研究品种的甘蔗中（E）-白藜芦醇和（E）-皮三醇含量都有决定性影响。相对湿度为60％时，其浓度高于70％。这是首次在受控的温度，时间和相对湿度条件下对修剪后储存的甘蔗的原花色素轮廓进行监测。在两种相对湿度下的储存过程中，（-）-表儿茶素的浓度均降低。此外，原花青素B1和原花青素二聚体的水平也有一定程度的降低。