A novel method for study the interaction between carbohydrate and lectin was developed in homogenous solution. DNA was introduced to the recognition process of carbohydrate with protein. Due to the versatile detection mode of DNA, the detection efficiency for carbohydrate and protein was highly promoted. Herein, two kinds of mannose-DNA conjugates were synthesized. One of which was used to prepare DNA-templated silver nanocluster (AgNCs) and the other one contained guanine-rich DNA sequences. When the mannose of two conjugates binding to lectin Concanavalin A (Con A), great enhancement on fluorescence intensity was obtained due to the proximity ligation of the DNA-templated Ag NCs with guanine-rich DNA sequences. Hence Con A can be quantified conveniently in homogenous solution using Ag NCs with low toxicity and tunable fluorescence. Moreover, carbohydrate functionalized DNA-templated AgNCs was also utilized for cancer cell imaging based on the recognition of mannose with mannose receptor on cancer cell MDA-MB-231.

在均相溶液中开发了一种研究碳水化合物与凝集素之间相互作用的新方法。将DNA引入到碳水化合物与蛋白质的识别过程中。由于DNA的检测方式多样，因此大大提高了碳水化合物和蛋白质的检测效率。在此，合成了两种甘露糖-DNA缀合物。其中一个用于制备以DNA为模板的DNA纳米银簇（AgNC），而另一个则包含富含鸟嘌呤的DNA序列。当两种缀合物的甘露糖与凝集素伴刀豆球蛋白A（Con A）结合时，由于DNA模板化的Ag NCs与富含鸟嘌呤的DNA序列的接近连接，荧光强度得到了极大的增强。因此，使用具有低毒性和可调荧光的Ag NCs，可以在均质溶液中方便地定量ConA。而且，