Abstract Aeromonas spp. are closely associated with food spoilage, and often enter the food chain through cross-contamination during processing from biofilms on food-contacted surfaces. The attachment abilities of two high-spoilage Aeromonas salmonicida isolates were compared. The sensitivities of these isolates in biofilm to four disinfectants, namely sodium hypochlorite, chlorine dioxide, acidic electrolyzed water (AEW) and neutral electrolyzed water (NEW), were assayed. The results indicated that isolate 1425 showed higher attachment ability as compared to isolate 1435 at initial attachment (30 min) under a short-term attachment scenario (5 h), reaching more than 5 log CFU/cm2. Under a long-term attachment scenario (7 days), isolate 1425 formed a mature biofilm after 5 days of incubation, with the highest observed cell numbers of nearly 9 log CFU/cm2. The dynamics of biofilm formation of isolate 1435 were relatively stable, with cell population ranging from 6.25 to 7 log CFU/cm2. The composition of the extracellular polymeric substances and the morphology of the mature biofilm were identified by Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) and Confocal Laser Scanning Microscopy (CLSM). The mature biofilm formed by isolate 1425 showed more resistance to tested disinfectants than that of isolate 1435. Sodium hypochlorite (200 mg/L) and AEW (40 mg/L) showed greater lethal effects than the other disinfectants. Moreover, 40 mg/L of AEW was as effective as 200 mg/L of sodium hypochlorite. These findings indicated that the biofilm formed by microorganisms associated with food spoilage and safety could not be ignored during food processing and that AEW may be a promising alternative to sodium hypochlorite for the elimination of such biofilms.

中文翻译：

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杀鲑气单胞菌分离物：对四种消毒剂的附着能力和敏感性

摘要气单胞菌属。与食品腐败密切相关，并经常通过食品接触表面的生物膜加工过程中的交叉污染进入食物链。比较了两种高腐败气单胞菌的附着能力。测定了生物膜中这些分离物对四种消毒剂的敏感性，即次氯酸钠、二氧化氯、酸性电解水 (AEW) 和中性电解水 (NEW)。结果表明，在短期附着情况（5 小时）下，与分离株 1435 相比，分离株 1425 在初始附着（30 分钟）时表现出更高的附着能力，达到超过 5 log CFU/cm2。在长期附着情况下（7 天），分离株 1425 在培养 5 天后形成成熟的生物膜，观察到的最高细胞数接近 9 log CFU/cm2。分离株 1435 的生物膜形成动力学相对稳定，细胞数量范围为 6.25 至 7 log CFU/cm2。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和共聚焦激光扫描显微镜（CLSM）鉴定细胞外聚合物的组成和成熟生物膜的形态。由分离株 1425 形成的成熟生物膜对测试消毒剂显示出比分离株 1435 更强的抗性。次氯酸钠 (200 mg/L) 和 AEW (40 mg/L) 显示出比其他消毒剂更大的致死作用。此外，40 mg/L 的 AEW 与 200 mg/L 的次氯酸钠一样有效。