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The protective role of multixenobiotic resistance (MXR)-mediated ATP-binding cassette (ABC) transporters in biocides-exposed rotifer Brachionus koreanus
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2017-12-30 , DOI: 10.1016/j.aquatox.2017.12.016
Young Hwan Lee , Hye-Min Kang , Min-Sub Kim , Jin-Sol Lee , Chang-Bum Jeong , Jae-Seong Lee

P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) are ATP-binding cassette (ABC) transporters that confer multixenobiotic resistance (MXR) via their efflux activity, which enables a variety of xenobiotics to be expelled from cells. MXR has been proposed as the first line of defense against xenobiotics. In this study, the protective roles of P-gp and MRP in the rotifer Brachionus koreanus were examined in response to four biocides (alachlor, chlorpyrifos, endosulfan, and molinate) using fluorescent substrates and inhibitors specific to P-gp and MRP. Exposure of rotifers to the four biocides resulted in increased P-gp and MRP activity. Moreover, the rotifers became more sensitive to the biocides with a reduced tendency in survival and slower population growth rates, when P-gp or MRP was inhibited. These findings suggest that P-gp and MRP are involved in the defense system in response to biocide exposure. Furthermore, the transcriptional levels of the genes encoding P-gp and MRP were examined to uncover the mechanism by which MXR is regulated. Our results demonstrate a crucial role of the MXR efflux system in the defense response to biocides, thereby providing a better understanding of rotifer defense mechanisms on the molecular level.



中文翻译:

多异源抗药性(MXR)介导的ATP结合盒(ABC)转运蛋白在暴露于杀生物剂的轮虫Brachionus koreanus中的保护作用

P-糖蛋白(P-gp)和多药抗性相关蛋白(MRP)是ATP结合盒(ABC)转运蛋白,可通过其外排活性赋予多异生物抗性(MXR),从而使多种异生物从细胞中排出。MXR已被提议作为针对异种生物的第一道防线。在这项研究中,P-gp和MRP在轮虫Brachionus koreanus中的保护作用使用荧光底物和对P-gp和MRP特异的抑制剂对四种杀生物剂(丙草胺,毒死rif,硫丹和,酸盐)的反应进行了检测。轮虫暴露于四种杀生物剂会导致P-gp和MRP活性增加。此外,当抑制P-gp或MRP时,轮虫对杀生物剂变得更敏感,具有降低的生存趋势和较慢的种群生长速度。这些发现表明,P-gp和MRP参与了对杀生物剂暴露的防御系统。此外,检查了编码P-gp和MRP的基因的转录水平,以揭示调节MXR的机制。我们的结果表明MXR外排系统在对生物杀伤剂的防御反应中起着至关重要的作用,

更新日期:2017-12-30
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