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Multixenobiotic resistance in Mytilus edulis: Molecular and functional characterization of an ABCG2- type transporter in hemocytes and gills
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2017-12-28 , DOI: 10.1016/j.aquatox.2017.12.012
Yosra Ben Cheikh , Benoit Xuereb , Céline Boulangé-Lecomte , Frank Le Foll

Among the cellular protection arsenal, ABC transporters play an important role in xenobiotic efflux in marine organisms. Two pumps belonging to B and C subfamily has been identified in Mytilus edulis. In this study, we investigated the presence of the third major subtype ABCG2/BCRP protein in mussel tissues. Transcript was expressed in hemocytes and with higher level in gills. Molecular characterization revealed that mussel ABCG2 transporter shares the sequence and organizational structure with mammalian and molluscan orthologs. Overall identity of the predicted amino acid sequence with corresponding homologs from other organisms was between 49% and 98%. Moreover, protein efflux activity was demonstrated using a combination of fluorescent allocrites and specific inhibitors. The accumulation of bodipy prazosin and pheophorbide A was heterogeneous in gills and hemocytes. Most of the used blockers enhanced probe accumulation at different levels, most significantly for bodipy prazosin. Moreover, Mrp classical blocker MK571 showed a polyspecificity.

In conclusion, our data demonstrate that several ABC transporters contribute to MXR phenotype in the blue mussel including ABCG2 that forms an active pump in hemocytes and gills. Efforts are needed to distinguish between the different members and to explore their single function and specificity towards allocrites and chemosensitizers.



中文翻译:

在多种异型生电阻紫贻贝:分子和在血细胞和鳃的ABCG2-型转运的功能表征

在细胞保护库中,ABC转运蛋白在海洋生物的异源外排中起重要作用。紫花苜蓿中鉴定出两个属于B和C亚科的泵。在这项研究中,我们调查了贻贝组织中第三主要亚型ABCG2 / BCRP蛋白的存在。转录本在血细胞中表达,在g中表达更高。分子表征显示,贻贝ABCG2转运蛋白与哺乳动物和软体动物的直系同源基因具有相同的序列和组织结构。预测的氨基酸序列与来自其他生物的相应同源物的总体同一性在49%至98%之间。此外,使用荧光金属榴石和特定抑制剂的组合证明了蛋白质外排活性。bodipy的prazosin和ophophorbide A的积累在g和血细胞中是异质的。大多数使用的阻滞剂可在不同水平上增强探针的积累,其中最明显的是对bodipy prazosin而言。而且,

总之,我们的数据表明,几种ABC转运蛋白有助于蓝贻贝中的MXR表型,包括在血细胞和g中形成主动泵的ABCG2。需要做出努力以区分不同的成员,并探索它们对单石和化学增敏剂的单一功能和特异性。

更新日期:2017-12-28
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