There have been ongoing efforts to develop more sensitive and fast quantitative screening of cancer markers by use of fluorometric immunochromatographic test strips (ICTS) since the remarkable advances in fluorescent nanomaterials. Semiconducting polymer dots (Pdots) have recently emerged as a new type of biocompatible fluorescent probe with extraordinary brightness which is suitable for biological and clinical use. Here, we developed Pdot-based ICTS for quantitative rapid screening of prostate-specific antigen (PSA), α-fetoprotein (AFP), and carcinoembryonic antigen (CEA) in 10 min. Through use of the ultrahigh fluorescence brightness of Pdots, this immunosensor enabled much better detection sensitivity (2.05, 3.30, and 4.92 pg/mL for PSA, AFP, and CEA, respectively), in which the detection limit is at least 2 orders of magnitude lower than that of conventional fluorometric ICTS. Furthermore, we performed proof-of-concept experiments for simultaneous determination of multiple tumor markers in a single test strip. These results demonstrated that this Pdot-based ICTS platform is a promising candidate for developing new generations of point-of-care diagnostics. To the best of our knowledge, this is the first example of Pdot-based ICTS with multiplexing capability.

中文翻译：

---

多色基于聚合物点的免疫层析试纸条用于肿瘤标志物的多重检测

自从荧光纳米材料的显着进步以来，一直在努力通过使用荧光免疫层析试纸（ICTS）开发更敏感和快速的癌症标志物定量筛选方法。半导体聚合物点（Pdots）最近已经成为一种新型的生物相容性荧光探针，具有非凡的亮度，适用于生物学和临床用途。在这里，我们开发了基于Pdot的ICTS，可在10分钟内定量快速筛查前列腺特异性抗原（PSA），甲胎蛋白（AFP）和癌胚抗原（CEA）。通过使用Pdots的超高荧光亮度，该免疫传感器实现了更好的检测灵敏度（对于PSA，AFP和CEA分别为2.05、3.30和4.92 pg / mL），其中检出限比常规的荧光ICTS检出限低至少2个数量级。此外，我们进行了概念验证实验，可同时确定单个测试条中的多个肿瘤标志物。这些结果表明，基于Pdot的ICTS平台是开发新一代即时医疗诊断系统的有前途的候选者。据我们所知，这是具有复用功能的基于Pdot的ICTS的第一个示例。