In this work, we report a highly sensitive colorimetric sensing strategy for cancer biomarker diagnosis using gold nanoparticles (AuNPs) labeled with biotinylated poly(adenine) ssDNA sequences and streptavidin-horseradish peroxidase for enzymatic signal enhancement. By adopting this DNA-AuNP nanoconjugate sensing strategy, we were able to eliminate the complicated and costly thiol-binding process typically used to modify AuNP surfaces with ssDNA. In addition, different antibodies can be introduced to the AuNP surfaced via electrostatic interactions to provide highly specific recognition sites for biomolecular sensing. Moreover, multiple, simultaneous tests can be rapidly performed with low sample consumption by incorporating these surface-modified AuNPs into a paper-based analytical device that can be read using just a smartphone. As a result of these innovations, we were able to achieve a detection limit of 10 pg/mL for a prostate specific antigen in a test that could be completed in as little as 15 min. These results suggest that the proposed paper platform possesses the capability for sensitive, high-throughput, and on-site prognosis in resource-limited settings.

中文翻译：

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信号放大金纳米颗粒在纸质分析仪上进行癌症诊断

在这项工作中，我们报告了一种高灵敏度的比色传感策略，用于使用生物素标记的聚腺嘌呤ssDNA序列和链霉亲和素-辣根过氧化物酶标记的金纳米颗粒（AuNPs）进行酶信号增强，从而诊断癌症生物标志物。通过采用这种DNA-AuNP纳米共轭物传感策略，我们能够消除通常用于用ssDNA修饰AuNP表面的复杂且昂贵的硫醇结合过程。另外，可以通过静电相互作用将不同的抗体引入到表面的AuNP上，以提供用于生物分子感测的高度特异性的识别位点。此外，通过将这些表面改性的AuNPs掺入仅可通过智能手机读取的纸质分析设备中，就可以以低样品消耗量快速执行多个同时测试。这些创新的结果是，我们能够在短短15分钟内完成的一项测试中，对前列腺特异抗原的检测限达到10 pg / mL。这些结果表明，所提出的论文平台具有在资源有限的环境中进行敏感，高通量和现场预后的能力。