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Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins†
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2017-12-27 00:00:00 , DOI: 10.1039/c7tb03001k
Xiao-Yu Sun 1, 2, 3, 4, 5 , Run-Tian Ma 1, 2, 3, 4, 5 , Juan Chen 1, 2, 3, 4, 5 , Yan-Ping Shi 1, 2, 3, 4, 5
Affiliation  

Many diseases ordinarily have several kinds of biomarkers (most of them belong to the glycoprotein family). Simultaneous capturing of multiple target glycoprotein biomarkers can obtain better specificity compared with single marker detection in early screening of many diseases. In this work, we attempted to prepare magnetic molecularly imprinted nanoparticles with multiple recognition sites. Horseradish peroxidase and ovalbumin as the frequently used glycoproteins for simulation of glycoprotein biomarkers were employed as dual-templates. The simultaneous preorganization of two templates was achieved by introducing 4-formylphenylboronic acid as a functional monomer. To gain a high adsorption capacity for the two templates, the mass ratio of the two templates was precisely investigated. Controllable polymerization of dopamine was applied to synthesize thin imprinted layers that reduce mass transfer resistance and improve the eluted efficiency of the two templates. The resultant magnetic molecularly imprinted nanoparticles exhibited high adsorption capacities of 34.09 and 51.18 mg g−1, respectively. The simultaneous recognition of two templates in spiked fetal bovine serum was also achieved with satisfactory selectivity. The results indicate that this proposed strategy is potentially applicable for simultaneously and selectively capturing several glycoprotein biomarkers used in detecting many diseases.

中文翻译:

具有多个识别位点的磁性分子印迹纳米颗粒的合成,用于同时和选择性捕获两种糖蛋白

许多疾病通常具有几种生物标志物(大多数属于糖蛋白家族)。在许多疾病的早期筛查中,与单个标记物检测相比,同时捕获多个靶糖蛋白生物标记物可以获得更好的特异性。在这项工作中,我们尝试制备具有多个识别位点的磁性分子印迹纳米颗粒。辣根过氧化物酶和卵清蛋白作为模拟糖蛋白生物标志物的常用糖蛋白被用作双模板。通过引入4-甲酰基苯基硼酸作为功能单体实现了两个模板的同时预组织。为了获得两个模板的高吸附容量,精确研究了两个模板的质量比。将多巴胺的可控聚合应用于合成薄的压印层,以降低传质阻力并提高两个模板的洗脱效率。所得的磁性分子印迹纳米颗粒显示出34.09和51.18 mg g的高吸附容量-1。同时以令人满意的选择性实现了对掺入胎牛血清中两种模板的同时识别。结果表明,该提议的策略潜在地适用于同时并选择性地捕获用于检测多种疾病的几种糖蛋白生物标记。
更新日期:2017-12-27
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