The pathway of vesicle adsorption onto a solid support depends on the material composition of the underlying support, and there is significant interest in developing material-independent strategies to modulate the spectrum of vesicle–substrate interactions on a particular surface. Herein, using the quartz crystal microbalance-dissipation (QCM-D) technique, we systematically investigated how solution pH and membrane surface charge affect vesicle adsorption onto a silicon dioxide surface. While vesicle adsorption and spontaneous rupture to form complete supported lipid bilayer (SLBs) occurred in acidic conditions, it was discovered that a wide range of adsorption pathways occurred in alkaline conditions, including (i) vesicle adsorption and spontaneous rupture to form complete SLBs, (ii) vesicle adsorption and spontaneous rupture to form incomplete SLBs, (iii) irreversible adsorption of intact vesicles, (iv) reversible adsorption of intact vesicles, and (v) negligible adsorption. In general, SLB formation became more favorable with increasingly positive membrane surface charge although there were certain conditions at which attractive electrostatic forces were insufficient to promote vesicle rupture. To rationalize these findings, we discuss how solution pH and membrane surface charge affect interfacial forces involved in vesicle–substrate interactions. Taken together, our findings present a comprehensive picture of how interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces and offer a broadly applicable framework to characterize the interactions between phospholipid vesicles and inorganic material surfaces.

中文翻译：

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界面力决定了磷脂囊泡在二氧化硅表面上的吸附途径

囊泡吸附到固体支持物上的途径取决于基础支持物的材料组成，因此人们对开发独立于材料的策略以调节特定表面上的囊泡-底物相互作用的光谱非常感兴趣。本文中，我们使用石英微平衡耗散（QCM-D）技术，系统地研究了溶液的pH值和膜表面电荷如何影响囊泡在二氧化硅表面的吸附。虽然在酸性条件下会发生囊泡吸附和自发破裂以形成完整的支持脂质双层（SLB），但发现在碱性条件下会发生多种吸附途径，包括（i）囊泡吸附和自发破裂以形成完整的SLB，（ii）囊泡吸附和自发破裂形成不完整的SLB，（iii）完整囊泡的不可逆吸附，（iv）完整囊泡的可逆吸附，和（v）吸附量可忽略不计。通常，尽管在某些情况下吸引静电力不足以促进囊泡破裂，但随着膜表面正电荷的增加，SLB的形成变得更加有利。为了合理化这些发现，我们讨论了溶液的pH值和膜表面电荷如何影响囊泡-底物相互作用中涉及的界面力。在一起