Herein, a carbon quantum dot-based highly selective luminescent probe has been designed for the detection of the pesticide flumioxazin via the alkyne azide click reaction. An azide (N₃)-functionalized QD has been synthesized by conjugating 4-azidoaniline on the surface of the CQD by carbodiimide coupling. The turn-on fluorescence of the probe upon the addition of flumioxazin is attributed to the formation of a triazole-functionalized CQD in the presence of Cu(II) and ascorbic acid. The sensing protocol was also successfully validated in a number of compounds containing alkynyl groups. The fluorescence increase factor for flumioxazin (F/F₀) works linearly in the concentration range 0–35.4 μg l⁻¹, and the limit of detection is found to be 0.027 μg l⁻¹. The specific recognition of alkynyl groups towards azide in the presence of Cu(II) makes our probe specific and selective towards flumioxazin even in the presence of other pesticides. The utility of the developed sensor has also been explored in real agricultural samples.

中文翻译：

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一种新型的基于碳量子点的荧光纳米传感器，用于选择性检测实际样品中的氟米嗪†

在本文中，已经设计了基于碳量子点的高选择性发光探针，用于通过炔叠氮点击反应检测农药氟米嗪。通过碳二亚胺偶联在CQD表面缀合4-叠氮苯胺可合成出叠氮化物（N ₃）-官能化的QD。加入氟米嗪后探针的开启荧光归因于在铜（II）和抗坏血酸存在下三唑官能化CQD的形成。该传感方案也已在许多含有炔基的化合物中得到成功验证。氟米沙星的荧光增加因子（F / F ₀）在0–35.4μgl的浓度范围内呈线性关系^-1，并且检测极限为0.027μgl ^-1。在Cu（ II）存在下对叠氮化物的炔基的特异性识别，即使在存在其他农药的情况下，也使我们的探针对氟米嗪具有特异性和选择性。已开发的传感器的实用性也在实际的农业样品中得到了探索。