The preventive role of a purified Ganoderma atrum polysaccharide PSG-1-F₂ as a new dietary antioxidant against the intestinal toxicity of acrylamide (ACR) was investigated in vitro. Our results showed that ACR could induce oxidative stress in IEC-6 cells by the overproduction of reactive oxygen species (ROS) and malondialdehyde (MDA), and as well as the reduction in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). In addition, the induction of a mitochondria-mediated intrinsic apoptotic pathway by ACR was evidenced by the events of loss of mitochondrial membrane potential, bax/bcl-2 dysregulation, cytochrome c release, and activation of caspase-3. Interestingly, PSG-1-F₂ was able to suppress ACR toxicity by improving the redox status of IEC-6 cells and by attenuating mitochondria-mediated apoptosis. Its protective effect was even superior to the clinically used antioxidant N-acetylcysteine (NAC). This study uniquely introduces PSG-1-F₂ as a potential inhibitor of ACR-induced stress and toxicities.

中文翻译：

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的保护作用灵芝atrum针对丙烯酰胺诱导的氧化损伤多糖经由线粒体介导的内在凋亡途径在IEC-6细胞†

体外研究了纯化的灵芝灵芝多糖PSG-1-F ₂作为新型饮食抗氧化剂对丙烯酰胺（ACR）肠道毒性的预防作用。我们的结果表明，ACR可以通过过量产生活性氧（ROS）和丙二醛（MDA）以及降低超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH）的活性来诱导IEC-6细胞的氧化应激-Px）。此外，线粒体膜电位丧失，bax / bcl-2失调，细胞色素c释放和caspase-3活化的事件证明了ACR诱导线粒体介导的内在凋亡途径。有趣的是，PSG-1-F ₂通过改善IEC-6细胞的氧化还原状态和减弱线粒体介导的细胞凋亡，能够抑制ACR毒性。它的保护作用甚至优于临床使用的抗氧化剂N-乙酰半胱氨酸（NAC）。这项研究独特地介绍了PSG-1-F ₂作为ACR诱导的压力和毒性的潜在抑制剂。