An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy. In this strategy, carboxyl poly(9,9-dioctyfluorenyl-2,7-diyl) dots (PFO dots) were worked as ECL donor and 3,4,9,10-perylenetetracar-boxylic acid (PTCA) exploited as ECL acceptor, and hydrogen peroxide (H₂O₂) employed as the coreactant. The ECL donor and ECL acceptor were separately labeled with primary antibody (Ab₁) and secondary antibody (Ab₂), forming a sensing interface to the analyte target, insulin. In this expected sandwich-type ECL biosensor, PFO dots acted as sensing platform and PTCA employed as labels to quench the ECL emission of PFO dots. During the determination process, ECL signal of PFO dots was decreased in a gradual way by the increase of insulin concentration, and the quenching mechanism was also investigated. Under the optimal experimental conditions, the constructed biosensor exhibited an excellent performance, including a wide linear range from 1.0 × 10⁻⁵ ng/mL to 1.0 × 10² ng/mL, low detection limit of 3.0 × 10⁻⁶ ng/mL, good stability and selectivity for the detection of insulin. This pair of PFO-PTCA, as a new donor-acceptor pair in ECL-RET system, would provide a promising platform for bioanalysis in ECL field.

设计了一种电化学发光（ECL）生物传感器，用于使用新型ECL共振能量转移（ECL-RET）策略确定胰岛素。在此策略中，羧基聚（9,9-二辛基芴基-2,7-二基）点（PFO点）被用作ECL供体，而3,4,9,10-per四甲酸-羧酸（PTCA）被用作ECL受体，用过氧化氢（H ₂ O ₂）作为共反应剂。ECL供体和ECL受体分别用一抗（Ab ₁）和二抗（Ab ₂）标记），形成与分析物目标胰岛素的传感界面。在这种预期的夹心型ECL生物传感器中，PFO点充当传感平台，而PTCA充当标签，以淬灭PFO点的ECL发射。在测定过程中，随着胰岛素浓度的增加，PFO点的ECL信号逐渐降低，并研究了猝灭机理。在最佳实验条件下，构建的生物传感器表现出出色的性能，包括从1.0×10 ^-5  ng / mL到1.0×10 ²  ng / mL的宽线性范围，低检测限3.0×10 ^-6 ng / mL，对胰岛素的检测具有良好的稳定性和选择性。这对PFO-PTCA作为ECL-RET系统中新的供体-受体对，将为ECL领域的生物分析提供有希望的平台。