Oxidative stress has been implicated in the development of many diseases including cancer. Curculigoside, a phenolic glycoside, has been reported to protect various models against damage induced by oxidative stress. However, the protective effect of curculigoside on oxidative stress in normal human breast cells remains unclear. This study evaluated whether curculigoside protected MCF 10A human breast epithelial cells from H₂O₂-induced cytotoxicity and explored its underlying mechanisms. The H₂O₂-induced oxidative stress of cell models was demonstrated by decreased percentages of cell viability, increased levels of ROS, reduced activities in antioxidant enzymes, and inhibition of the expressions of antioxidant proteins. More importantly, pre-treatment with curculigoside significantly inhibited the H₂O₂-induced apoptosis in this cell model. Together, curculigoside significantly prevented the cytotoxic effects of H₂O₂, suggesting that it protected the cells against H₂O₂-induced oxidative damage. Our findings provide new insight into the cytoprotective effects of curculigoside and the possible mechanisms underlying these effects.

氧化应激与包括癌症在内的许多疾病的发展有关。仙茅苷，一种酚类糖苷，据报道可以保护各种模型免受氧化应激引起的损害。然而，仙茅苷对正常人乳腺细胞氧化应激的保护作用仍不清楚。这项研究评估了仙茅苷是否能保护MCF 10A人乳腺上皮细胞免受H ₂ O ₂诱导的细胞毒性作用，并探讨了其潜在机制。H ₂ O ₂细胞活力的百分比降低，ROS水平升高，抗氧化酶活性降低以及抗氧化蛋白表达受到抑制，证明了细胞模型诱导的氧化应激。更重要的是，在该细胞模型中，用仙茅苷预处理可以显着抑制H ₂ O ₂诱导的细胞凋亡。一起，仙茅苷可显着阻止H ₂ O ₂的细胞毒性作用，表明它可保护细胞免受H ₂ O ₂诱导的氧化损伤。我们的发现为仙茅苷的细胞保护作用及其潜在机制提供了新的见解。