A Gram-positive bacterium, Rhodococcus wratislaviensis strain 9, was isolated from groundwater contaminated with nitrophenolics and trichloroethene following enrichment culture technique. The cells of strain 9 grown on LB broth (uninduced) degraded 720 µM p-nitrophenol (PNP) within 12 h, and utilized as a source of carbon and energy. Orthogonal experimental design analysis to determine optimal conditions for biodegradation of PNP showed that pH had a significant positive effect (P ≤ 0.05) on bacterial degradation of PNP, while glucose, di- and tri-nitrophenols exhibited significant negative effect. Cell-free extracts obtained from PNP-grown culture that contained 20 µg mL^-1 protein degraded 90% of 720 µM PNP within 5 h of incubation. Two-dimensional protein analysis revealed differential expression of the oxygenase component of PNP monooxygenase and an elongation factor Tu in PNP-grown cells, but not in those grown on glucose. The strain 9 remediated laboratory wastewater containing 900 µM PNP efficiently within 14 h, indicating its great potential in bioremediation of PNP-contaminated waters.

通过富集培养技术，从被硝基酚和三氯乙烯污染的地下水中分离出革兰氏阳性细菌wratislaviensis菌株9。在LB肉汤应变9生长的（未诱导）的细胞退化720 μ中号p 12小时内硝基苯酚（PNP），并且作为碳和能量的来源使用。正交试验设计分析，以确定对PNP的生物降解最佳条件表明，pH值有显著正效应（P上PNP的细菌降解≤0.05），而葡萄糖，二和三硝基酚表现出显著负面影响。从PNP生长的培养物中获得的无细胞提取物，其中含有20 µg mL ^-1在孵育5小时内，蛋白质降解了720 µ M PNP的90％。二维蛋白质分析显示，在PNP生长的细胞中，PNP单加氧酶的加氧酶成分和延伸因子Tu有差异表达，而在葡萄糖上生长的细胞中则无差异。含900菌株9修复的实验室废水μ中号PNP内14小时有效，表明PNP污染海域的生物修复其巨大潜力。