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Rotational paper-based electrochemiluminescence immunodevices for sensitive and multiplexed detection of cancer biomarkers
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-05-01 , DOI: 10.1016/j.aca.2017.12.005
Xiange Sun 1 , Bowei Li 2 , Chunyuan Tian 3 , Fabiao Yu 2 , Na Zhou 2 , Yinghua Zhan 4 , Lingxin Chen 2
Affiliation  

This paper describes a novel rotational paper-based analytical device (RPAD) to implement multi-step electrochemiluminescence (ECL) immunoassays. The integrated paper-based rotational valves can be easily controlled by rotating paper discs manually and this advantage makes it user-friendly to untrained users to carry out the multi-step assays. In addition, the rotational valves are reusable and the response time can be shortened to several seconds, which promotes the rotational paper-based device to have great advantages in multi-step operations. Under the control of rotational valves, multi-step ECL immunoassays were conducted on the rotational device for the multiplexed detection of carcinoembryonic antigen (CEA) and prostate specific antigen (PSA). The rotational device exhibited excellent analytical performance for CEA and PSA, and they could be detected in the linear ranges of 0.1-100 ng mL-1 and 0.1-50 ng mL-1 with detection limits down to 0.07 ng mL-1 and 0.03 ng mL-1, respectively, which were within the ranges of clinical concentrations. We hope this technique will open a new avenue for the fabrication of paper-based valves and provide potential application in clinical diagnostics.

中文翻译:

用于敏感和多重检测癌症生物标志物的旋转纸基电化学发光免疫装置

本文介绍了一种新型旋转纸基分析装置 (RPAD),以实现多步电化学发光 (ECL) 免疫测定。集成的纸基旋转阀可以通过手动旋转纸盘轻松控制,这一优势使得未经培训的用户可以方便地进行多步骤检测。此外,旋转阀可重复使用,响应时间可缩短至数秒,这使得旋转纸基装置在多步骤操作中具有很大优势。在旋转阀的控制下,在旋转装置上进行多步 ECL 免疫测定,用于癌胚抗原 (CEA) 和前列腺特异性抗原 (PSA) 的多重检测。旋转装置对 CEA 和 PSA 表现出优异的分析性能,它们可以在 0.1-100 ng mL-1 和 0.1-50 ng mL-1 的线性范围内被检测到,检测限分别低至 0.07 ng mL-1 和 0.03 ng mL-1,均在临床浓度。我们希望这项技术将为纸基瓣膜的制造开辟一条新途径,并在临床诊断中提供潜在的应用。
更新日期:2018-05-01
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