当前位置: X-MOL 学术J. Am. Chem. Soc. › 论文详情
Modular Protein Cages for Size-Selective RNA Packaging in Vivo
Journal of the American Chemical Society ( IF 14.357 ) Pub Date : 2018-01-03 , DOI: 10.1021/jacs.7b10798
Yusuke Azuma, Thomas G. W. Edwardson, Naohiro Terasaka, Donald Hilvert

Protein cages have recently emerged as an important platform for nanotechnology development. Of the naturally existing protein cages, viruses are among the most efficient nanomachines, overcoming various barriers to achieve component replication and efficient self-assembly in complex biological milieu. We have designed an artificial system that can carry out the most basic steps of viral particle assembly in vivo. Our strategy is based on patchwork capsids formed from Aquifex aeolicus lumazine synthase and a circularly permuted variant with appended cationic peptides. These two-component protein containers self-assemble in vivo, capturing endogenous RNA molecules in a size-selective manner. By varying the number and design of the RNA-binding peptides displayed on the lumenal surface, the length of guest RNA can be further controlled. Using a fluorescent aptamer, we also show that short-lived RNA species are captured by the protein cage. This platform has potential as a model system for investigating virus assembly, as well as developing RNA regulation or sampling tools to augment biotechnology.
更新日期:2018-01-03

 

分享到
评论: 0
期刊列表
广州大学胡春教授团队招聘科研秘书和特聘工程师
深圳格拉布斯研究院研究序列教授和工程师招聘启事
北京理工大学青年千人课题组联合招聘硕士生、博士生以及博士后人员(合成化学和材料方向)
天合科研
肠道菌群相关课题设计及研究策略专题会议
会议通知 | ACS全球科技研讨会:化学与生命科学前沿
【问答】请问如何表征金属纳米颗粒表面的缺陷?
2017年中科院JCR分区化学大类列表
试剂库存管理
化合物查询
down
wechat
bug