The isomerization of sucrose to isomaltulose for the production of isomalt using the enzyme sucrose isomerase (SI) [EC5.4.99.11] is a known industrial process preferably using immobilized biocatalysts. We describe a novel immobilization method that has been developed to overcome disadvantages of current immobilization techniques and to meet todaýs industrial requirements. Current methods are primarily very process specific and often suffer from scale-up limitations, thus preventing their industrial use. We demonstrate, that industrially already established processes can be replaced by cost-effective formulation technologies comprising of only a few process steps for producing the heterogeneous biocatalyst. Feasibility studies simulating industrial process conditions show that the granules provide good activity and sucrose conversion enabling their application in a fixed-bed reactor.

使用蔗糖异构酶（SI）[EC5.4.99.11]将蔗糖异构化为异麦芽酮糖是用于生产异麦芽酮糖的一种已知工业方法，优选使用固定化生物催化剂。我们描述了一种新颖的固定方法，该方法已被开发来克服当前固定技术的缺点并满足todaýs工业要求。当前的方法主要是非常特定于过程的，并且经常遭受规模扩大的局限，从而阻止了其工业应用。我们证明，工业上已经建立的过程可以由经济有效的配方技术代替，该技术仅包含用于生产非均相生物催化剂的几个工艺步骤。