Abstract Tauroursodeoxycholic acid (TUDCA) has a role in the regulation of the differentiation of mesenchymal stem cells. In this study, TUDCA was demonstrated to increase osteogenic differentiation at a low concentration (25 μM) and chondrogenic differentiation at a high concentration (2500 μM). We investigated the regeneration of osteochondral defects in a rat model using a bilayer plug loaded with two different concentration of TUDCA-PLGA microspheres (TUDCA-MS bilayer plug). The TUDCA-MS bilayer plug successfully regenerated cartilage and subchondral bone layer. Therefore, we suggest that the TUDCA-MS bilayer plug as a new treatment method for simultaneous regeneration of cartilage and subchondral bone layer.

中文翻译：

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双层透明质酸水凝胶塞与 TUDCA-PLGA 微球修复大鼠关节骨软骨缺损

摘要 牛磺熊去氧胆酸（TUDCA）具有调节间充质干细胞分化的作用。在这项研究中，TUDCA 被证明可以在低浓度 (25 μM) 和高浓度 (2500 μM) 时增加成骨分化。我们使用加载有两种不同浓度的 TUDCA-PLGA 微球（TUDCA-MS 双层塞）的双层塞研究了大鼠模型中骨软骨缺损的再生。TUDCA-MS 双层插头成功地再生了软骨和软骨下骨层。因此，我们建议将 TUDCA-MS 双层塞作为一种新的治疗方法，用于同时再生软骨和软骨下骨层。